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Abstract：
Introduction Low birth weight (LBW), which is a risk factor for noncommunicable diseases throughout life, is a significant public health concern. In addition to regulating myeloid cell differentiation and proliferation, a transcriptional repressor identified as growth factor independence-1 (GFI-1) is essential for hematopoietic stem cell maintenance and self-renewal. The current study was designed to compare the expression of the GFI-1 gene in the differentiation of hematopoietic stem cells in newborns with LBW and those with normal birth weight (NBW). Methods A prospective comparative analytical study was carried out from September 2019 to September 2021 after obtaining Institute Ethical Committee approval at a tertiary care center in north India. The GFI-1 gene expression levels in 50 cord blood samples from women with term gestation and LBW newborns (<2500 grams) were measured using quantitative real-time polymerase chain reaction (RT-PCR) and compared to gene expression levels in 50 cord blood samples from women with term gestation and NBW newborns (≥2500 grams). The data were analyzed using IBM SPSS statistics software version 24.0 (IBM Corp., Armonk, NY). Results The median GFI-1 expression in LBW newborns is 3.1, whereas among NBW newborns it is 9.39. The difference is significant (P <0.001). The level of GFI-1 gene expression in LBW newborns was correlated with their birth weight. The coefficient of correlation was found to be weakly positive (r = 0.223). The birth weight of NBW newborns was correlated to the level of expression of the GFI-1 gene, which was found to be positively correlated (r = 0.332). Conclusion The levels of the GFI-1 gene and newborn birth weight were compared in LBW infants, which were weakly positively correlated. The level of GFI-1 gene expression at birth was compared to the birth weight of NBW newborns, which was positively correlated.
摘要：
低出生体重(LBW)这是非传染性疾病终生的危险因素，是一个重大的公共卫生问题。除了调节骨髓细胞的分化和增殖，被鉴定为生长因子独立性-1(GFI-1)的转录抑制因子对于造血干细胞维持和自我更新至关重要.本研究旨在比较GFI-1基因在LBW新生儿和正常出生体重（NBW）新生儿造血干细胞分化中的表达。方法在印度北部的三级护理中心获得研究所伦理委员会的批准后，于2019年9月至2021年9月进行了一项前瞻性比较分析研究。使用定量实时聚合酶链反应（RT-PCR）测量了50例足月妊娠和LBW新生儿（<2500克）的脐带血样本中的GFI-1基因表达水平，并与50例足月妊娠和NBW新生儿（≥2500克）的脐带血样本中的基因表达水平进行了比较。使用IBMSPSS统计软件24.0版(IBMCorp.,Armonk,NY).结果GFI-1在LBW新生儿中的表达中位数为3.1，而在NBW新生儿中的表达中位数为9.39。差异具有显著性(P<0.001)。LBW新生儿GFI-1基因表达水平与出生体重相关。发现相关系数为弱正（r=0.223）。NBW新生儿的出生体重与GFI-1基因的表达水平相关,呈正相关(r=0.332)。结论GFI-1基因水平与新生儿出生体重比较,呈弱正相关。将出生时GFI-1基因表达水平与NBW新生儿的出生体重进行比较，呈正相关。
