Abstract：
The present study describes the preparation of woven silk fabric (WSF) and poly(ethylene glycol) diacrylate (PEGDA) hydrogel composite reinforced artificial heart valve (SPAHV). Interestingly, the longitudinal and latitudinal elastic modulus of the SPAHV composite can achieve at 54.08 ± 3.29 MPa and 23.96 ± 2.18 MPa, respectively, while its volume/mass swelling ratio and water permeability was 1.9 %/2.8 % and 3 mL/(cm2∙min), respectively, revealing remarkable anisotropic mechanical properties, low water swelling property and water permeability. The in vitro & in vivo biocompatibility and anti-calcification ability of SPAHV were further examined using L929 mouse fibroblasts and Sprague Dawley (SD) male rat model under 8 weeks of subcutaneous implantation. The expression of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 was determined by immunohistochemical staining, as well as the H&E staining and alizarin red staining were accessed. The results showed that the composites possess better biocompatibility, resistance to degradation and anti-calcification ability compared to the control group (p < 0.05). Thus, the SPAHV composite with robust mechanical properties and biocompatibility has potential application for artificial heart valves.
摘要：
本研究描述了编织丝织物（WSF）和聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶复合材料增强的人工心脏瓣膜（SPAHV）的制备。有趣的是,SPAHV复合材料的纵向和横向弹性模量可以达到54.08±3.29MPa和23.96±2.18MPa，分别,而其体积/质量溶胀率和透水性为1.9％/2.8％和3mL/（cm2·min），分别,揭示了显著的各向异性机械性能，低水膨胀性能和透水性。使用L929小鼠成纤维细胞和SpragueDawley（SD）雄性大鼠模型在皮下植入8周下进一步检查了SPAHV的体外和体内生物相容性和抗钙化能力。免疫组化染色检测促炎细胞因子TNF-α和抗炎细胞因子IL-10的表达,以及H&E染色和茜素红染色。结果表明,复合材料具有较好的生物相容性,抗钙化能力与对照组比较(p<0.05)。因此，SPAHV复合材料具有坚固的机械性能和生物相容性，在人工心脏瓣膜中具有潜在的应用前景。
