Abstract：
Infiltration of immune cells into the tumor is one of the major drivers of antitumor immune response, which can direct the outcome of anticancer therapies. In mice, implantation of dorsal skinfold window chamber (DSWC) combined with intravital confocal fluorescence microscopy allows real-time observation of splenocyte extravasation and infiltration into tumors. Here, we describe a detailed procedure of the DSWC implantation, splenocyte isolation and fluorescent labeling, intravenous injection of labeled splenocytes, and imaging of splenocyte extravasation into tumors using confocal fluorescence microscopy.
摘要：
免疫细胞浸润肿瘤是抗肿瘤免疫应答的主要驱动因素之一,可以指导抗癌治疗的结果。在老鼠身上,背侧皮褶窗腔（DSWC）的植入与活体共聚焦荧光显微镜相结合，可以实时观察脾细胞的外渗和浸润到肿瘤中。这里,我们描述了DSWC植入的详细程序，脾细胞分离和荧光标记，静脉注射标记的脾细胞，和使用共聚焦荧光显微镜对脾细胞外渗到肿瘤中的成像。
