PDF(Pubmed)
Abstract：
This study investigates the potential of using bacteriophages to control foodborne pathogen biofilms on stainless steel surfaces in the food industry. Biofilm-forming bacteria can attach to stainless steel surfaces, rendering them difficult to eradicate even after a thorough cleaning and sanitizing procedures. Bacteriophages have been proposed as a possible solution, as they can penetrate biofilms and destroy bacterial cells within, reducing the number of viable bacteria and preventing the growth and spread of biofilms. This systematic review and meta-analysis evaluates the potential of bacteriophages against different biofilm-forming foodborne bacteria, including Cronobacter sakazakii, Escherichia coli, Staphylococcus aureus, Pseudomonas fluorescens, Pseudomonas aeruginosa and Listeria monocytogenes. Bacteriophage treatment generally causes a significant average reduction of 38 % in biofilm formation of foodborne pathogens on stainless steel. Subgroup analyses revealed that phages are more efficient in long-duration treatment. Also, applying a cocktail of phages is 1.26-fold more effective than applying individual phages. Phages at concentrations exceeding 107 PFU/ml are significantly more efficacious in eradicating bacteria within a biofilm. The antibacterial phage activity decreases substantially by 3.54-fold when applied at 4 °C compared to temperatures above 25 °C. This analysis suggests that bacteriophages can be a promising solution for controlling biofilms in the food industry.
摘要：
这项研究调查了在食品工业中使用噬菌体控制不锈钢表面食源性病原体生物膜的潜力。生物膜形成细菌可以附着在不锈钢表面，即使经过彻底的清洁和消毒程序，它们也很难根除。噬菌体已被提议作为一种可能的解决方案，因为它们可以穿透生物膜并破坏其中的细菌细胞，减少活细菌的数量，防止生物膜的生长和扩散。本系统综述和荟萃分析评估了噬菌体对不同生物膜形成食源性细菌的潜力。包括SakazakiiCronobacter,大肠杆菌,金黄色葡萄球菌,荧光假单胞菌,铜绿假单胞菌和单核细胞增生李斯特菌。噬菌体处理通常导致不锈钢上食源性病原体的生物膜形成显著平均减少38%。亚组分析显示，噬菌体在长期治疗中更有效。此外，使用噬菌体混合物比使用单个噬菌体有效1.26倍。浓度超过107PFU/ml的噬菌体在根除生物膜内的细菌方面显著更有效。与高于25°C的温度相比，在4°C下施用时，抗菌噬菌体活性大大降低了3.54倍。该分析表明，噬菌体可以成为食品工业中控制生物膜的有前途的解决方案。
