PDF(Pubmed)
Abstract：
Diabetic ketoacidosis (DKA) is regarded to be a communal complication of both type 1 and type 2 diabetes mellitus in children and adolescents. Successful therapy of DKA in children requires prompt diagnosis, strict monitoring of medical indicators, and prompt action. Thymoquinone (Tq) from black cumin loaded chitosan nanoparticles (ChNPs) intend to assess an effective agent to overcome this problem. XRD, FTIR, SEM, and TEM were used in the physicochemical analysis. Enzymatic activity of α-amylase and α-glucosidase was used in in vitro tests of anti-diabetic efficacy. Protecting insulin against enzyme breakdown is a crucial part of the insulin delivery mechanism. In the STZ-induced diabetes RIN-5F cell line, the anti-apoptotic capability of Tq-ChNPs was demonstrated through the NF-κB mediated apoptotic pathway. The combination of thymoquinone and chitosan NPs demonstrated that a wide variety of incredibly effective substances to elevate their curative effects, thus contributing to the growth of clinical and pharmaceutical fields.
摘要：
糖尿病酮症酸中毒（DKA）被认为是儿童和青少年1型和2型糖尿病的公共并发症。儿童DKA的成功治疗需要及时诊断，严格监控医疗指标，并迅速采取行动。来自黑色孜然负载壳聚糖纳米颗粒(ChNP)的胸腺醌(Tq)旨在评估克服该问题的有效试剂。XRD,FTIR,SEM,和TEM用于理化分析。α-淀粉酶和α-葡萄糖苷酶的酶活性用于抗糖尿病疗效的体外测试。保护胰岛素抵抗酶分解是胰岛素递送机制的关键部分。在STZ诱导的糖尿病RIN-5F细胞系中，通过NF-κB介导的凋亡途径证明了Tq-ChNPs的抗凋亡能力。百里香醌和壳聚糖NPs的组合表明，各种令人难以置信的有效物质可以提高其疗效，从而促进了临床和制药领域的发展。
