PDF(Pubmed)
Abstract：
Antibodies are one of the most used reagents in scientific laboratories and are critical components for a multitude of experiments in physiology research. Over the past decade, concerns about many biological methods, including those that use antibodies, have arisen as several laboratories were unable to reproduce the scientific data obtained in other laboratories. The lack of reproducibility could be largely attributed to inadequate reporting of detailed methods, no or limited verification by authors, and the production and use of unvalidated antibodies. The goal of this guideline article is to review best practices concerning commonly used techniques involving antibodies, including immunoblotting, immunohistochemistry, and flow cytometry. Awareness and integration of best practices will increase the rigor and reproducibility of these techniques and elevate the quality of physiology research.
摘要：
抗体是科学实验室中最常用的试剂之一，并且是生理学研究中许多实验的关键成分。在过去的十年里，对许多生物学方法的担忧，包括那些使用抗体的，由于许多实验室无法复制其他实验室获得的科学数据而出现。虽然一些缺乏可重复性可能是由于详细方法的报告不足，使用未经验证的抗体被确定为缺乏可重复性的主要结果来源.本指南文章的目的是回顾当前的使用情况，并提供有关涉及抗体的常用技术的最佳实践指南。包括免疫印迹,免疫组织化学，和流式细胞术。这些实践的知识和使用将增加这些技术的严谨性和可重复性，并提高生理学研究的质量。
