PDF(Pubmed)
Abstract：
Burkholderia mallei is the main cause of glanders as a dangerous contagious zoonosis disease that is mostly observed in single-hoofed animals, especially horses. Modern molecular techniques have been recently employed to improve epidemiology for identifying and searching for strains of this bacterium at different times and locations. Due to the unknown number of circulating strains and lack of preventive methods, glanders is still observed in the form of epidemics. The present study aimed to evaluate six field isolates plus two laboratory strains of Borkolderia mallei and Burkholderia pseudomallei using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. All the isolates and strains were microbially cultured in the glycerol nutrient and glycerol agar media. The individually grown colonies of the bacterium were used in the biochemical tests. The DNA of isolates was extracted by boiling, and the PCR-RFLP test was conducted on their genome. Finally, the bacterium was injected into guinea pigs to induce the Straus reaction. The biochemical assays (or bioassays) confirmed the isolates as Burkholderia mallei. The PCR-RFLP assay demonstrated a product for Burkholderia mallei with a length of 650 bp. Nevertheless, 250 and 400 bp were produced for Burkholderia pseudomallei. The swollen scrotum pointed to the occurrence of the Straus reaction. The PCR-RFLP is a proper differential diagnosis technique for B. mallei; moreover, it is a suitable method for differentiating between Burkholderia mallei and Burkholderia pseudomallei. This technique can detect Burkholderia mallei in a short time with high precision and sensitivity.
摘要：
伯克霍尔德氏菌是腺体作为一种危险的传染性人畜共患病的主要原因，主要在单蹄动物中观察到，尤其是马。最近已采用现代分子技术来改善流行病学，以在不同时间和位置鉴定和搜索该细菌的菌株。由于循环菌株数量不详，缺乏预防方法，腺体仍然以流行病的形式被观察到。本研究旨在使用聚合酶链反应限制性片段长度多态性（PCR-RFLP）方法评估六个野外分离株以及两个实验室菌株。将所有分离物和菌株在甘油营养素和甘油琼脂培养基中进行微生物培养。单独生长的细菌菌落用于生化测试。分离物的DNA是通过煮沸提取的，并对其基因组进行PCR-RFLP检测。最后,将该细菌注射到豚鼠中以诱导Straus反应。生化测定（或生物测定）证实了分离物是伯克霍尔德氏菌。PCR-RFLP分析证明了一种长度为650bp的伯克霍尔德氏菌产品。然而，假伯克霍尔德菌产生250和400bp。阴囊肿胀表明Straus反应的发生。PCR-RFLP是一种正确的鉴别诊断技术。此外，这是区分伯克霍尔德菌和假伯克霍尔德菌的合适方法。该技术可以在短时间内检测出马伯克霍尔德菌，具有较高的精度和灵敏度。
