Abstract：
Hyperbaric oxygen therapy (HBOT) has proven successful in wound healing. However, its potential effects on anterior cruciate ligament (ACL) injuries remain uncertain. This study aimed to investigate the impact of HBOT on graft healing following ACL reconstruction in rabbits. Male New Zealand rabbits underwent ACL reconstruction and were randomly divided into two groups: the HBOT group and the ambient air group. The HBOT group received 100% oxygen at 2.5 atmospheres absolute for 2 h daily for 5 consecutive days, starting from the first day after surgery. The ambient air group was maintained in normal room air throughout the entire period. After 12 weeks following the surgery, animals were euthanized, and their knees were harvested for analysis. The HBOT group demonstrated superior graft maturation and integration in comparison to the ambient air group, as evidenced by lower graft signal intensity on magnetic resonance imaging, decreased femoral and tibial tunnel size, and higher bone mineral density values on high-resolution peripheral quantitative computed tomography scans. Additionally, biomechanical testing indicated that the HBOT group had greater load to failure and stiffness values than the ambient air group. In conclusion, the adjuvant use of HBOT improved ACL graft maturation and integration, reduced tunnel widening, and enhanced the biomechanical properties of the graft. These results may provide important insights into the potential clinical application of HBOT as a therapeutic intervention to enhance graft healing after ACL reconstruction, paving the way for further research in this area.
摘要：
高压氧疗法（HBOT）已被证明在伤口愈合方面是成功的。然而,其对前交叉韧带(ACL)损伤的潜在影响仍不确定.本研究旨在探讨HBOT对兔ACL重建后移植物愈合的影响。雄性新西兰兔进行ACL重建，随机分为两组：HBOT组和环境空气组。HBOT组在2.5个绝对大气压下每天2小时接受100%氧气，连续5天，从手术后的第一天开始。环境空气组在整个期间保持在正常的室内空气中。手术后12周,动物被安乐死，并收集他们的膝盖进行分析。与环境空气组相比，HBOT组表现出优越的移植物成熟和整合，磁共振成像的移植物信号强度较低，股骨和胫骨隧道尺寸减小，和更高的骨密度值在高分辨率的外周定量计算机断层扫描。此外,生物力学测试表明，HBOT组比环境空气组具有更大的破坏载荷和刚度值。总之,HBOT的佐剂使用改善了ACL移植物的成熟和整合，减少隧道加宽，并增强了移植物的生物力学特性。这些结果可能为HBOT作为治疗干预以增强ACL重建后的移植物愈合的潜在临床应用提供重要见解。为这一领域的进一步研究铺平了道路。
