Abstract：
OBJECTIVE: Microscopic diagnosis of Giemsa-stained thick and thin blood films remained the gold standard laboratory method for the diagnosis of malaria. In this context, we felt it was important to conduct this evaluation with 40 public medical biology laboratories (MBLs) in the Abidjan 1 health region that perform blood parasitology tests to improve their implementation process.
METHODS: This descriptive and analytical study took place in July 2020 and involved participating laboratories (PLs) from the public sector in Abidjan. A set of 3 blood smear slides of variable parasite densities (PDs) with assigned values (AVs) of parasite densities and assigned Plasmodium species was used. The criterion for establishing the parasite density compliance interval was assigned values of ± 25%, and the performance rates were compared to the 80% recommended by the WHO for the African region.
RESULTS: Nearly a quarter (11/40) of the participating laboratories had a compliance rate greater than 80%, including 10 with a performance of 100% for the ability to identify parasites. Regarding identifying plasmodial species, a concordance rate of 100% was obtained for slide 1 for Plasmodium falciparum, while this rate was 20% for slide 2 for Plasmodium ovale. For parasite densities < 200/µl, 87.5% of the participating laboratories (PLs) had a performance rate lower than 80%, while 95% of these PLs had a performance rate higher than 80% for parasitaemia > 2000/µl.
CONCLUSIONS: There is a need to strengthen adapted to low parasitaemia, to improve the biological confirmation of malaria in Côte d\'Ivoire.
METHODS: This descriptive and analytical study took place in July 2020 and involved participating laboratories (PLs) from the public sector in Abidjan. A set of 3 blood smear slides of variable parasite densities (PDs) with assigned values (AVs) of parasite densities and assigned Plasmodium species was used. The criterion for establishing the parasite density compliance interval was assigned values of ± 25%, and the performance rates were compared to the 80% recommended by the WHO for the African region.
RESULTS: Nearly a quarter (11/40) of the participating laboratories had a compliance rate greater than 80%, including 10 with a performance of 100% for the ability to identify parasites. Regarding identifying plasmodial species, a concordance rate of 100% was obtained for slide 1 for Plasmodium falciparum, while this rate was 20% for slide 2 for Plasmodium ovale. For parasite densities < 200/µl, 87.5% of the participating laboratories (PLs) had a performance rate lower than 80%, while 95% of these PLs had a performance rate higher than 80% for parasitaemia > 2000/µl.
CONCLUSIONS: There is a need to strengthen adapted to low parasitaemia, to improve the biological confirmation of malaria in Côte d\'Ivoire.
摘要：
目的：Giemsa染色厚薄血膜的显微诊断仍然是诊断疟疾的金标准实验室方法。在这种情况下，我们认为,在阿比让1健康区域的40个公共医学生物学实验室(MBL)中进行这项评估非常重要,这些实验室进行血液寄生虫学检测,以改进其实施过程.
方法：这项描述性和分析性研究于2020年7月进行，涉及阿比让公共部门的参与实验室（PL）。使用了一组3个可变寄生虫密度（PD）的血涂片载玻片，其中具有寄生虫密度的分配值（AV）和分配的疟原虫物种。建立寄生虫密度顺应性区间的标准被指定为±25%，并将绩效率与世卫组织为非洲地区推荐的80%进行比较。
结果：近四分之一（11/40）的参与实验室的符合率超过80％，包括10个，具有100%的识别寄生虫的能力。关于鉴定疟原虫物种，对于恶性疟原虫，载玻片1的一致率为100％，而卵形疟原虫2号载玻片的这一比率为20%。对于寄生虫密度<200/µl，87.5%的参与实验室(PLs)的绩效率低于80%,而这些PLs中95%的寄生虫血症>2000/μl的表现率高于80%。
结论：有必要加强适应低寄生虫血症，以改善科特迪瓦疟疾的生物学确认。
方法：这项描述性和分析性研究于2020年7月进行，涉及阿比让公共部门的参与实验室（PL）。使用了一组3个可变寄生虫密度（PD）的血涂片载玻片，其中具有寄生虫密度的分配值（AV）和分配的疟原虫物种。建立寄生虫密度顺应性区间的标准被指定为±25%，并将绩效率与世卫组织为非洲地区推荐的80%进行比较。
结果：近四分之一（11/40）的参与实验室的符合率超过80％，包括10个，具有100%的识别寄生虫的能力。关于鉴定疟原虫物种，对于恶性疟原虫，载玻片1的一致率为100％，而卵形疟原虫2号载玻片的这一比率为20%。对于寄生虫密度<200/µl，87.5%的参与实验室(PLs)的绩效率低于80%,而这些PLs中95%的寄生虫血症>2000/μl的表现率高于80%。
结论：有必要加强适应低寄生虫血症，以改善科特迪瓦疟疾的生物学确认。
