Abstract：
Clogmia albipunctata (Williston, 1893) is a non-hematophagous insect belonging to the order Diptera, suborder Nematocera (Lower Diptera) and family Psychodidae. In the present work, we investigated how C. albipunctata control their midgut pH under different physiological conditions, comparing their midgut physiology with some nematoceran hematophagous species. The C. albipunctata midgut pH was measured after ingestion of sugar, protein and under the effect of the alkalinizing hormone released in the hemolymph of the hematophagous sand fly Lutzomyia longipalpis obtained just after a blood meal. The midgut pH of unfed or sugar-fed C. albipunctata is 5.5-6, and its midgut underwent alkalinization after protein ingestion or under treatment with hemolymph collected from blood fed L. longipalpis. These results suggested that in nematocerans, mechanisms for pH control seem shared between hematophagous and non-hematophagous species. This kind of pH control is convenient for successful blood digestion. The independent evolution of many hematophagous groups from the Lower Diptera suggests that characteristics involved in midgut pH control were already present in non-hematophagous species and represent a readiness for adaptation to this feeding mode.
摘要：
Clogmiaalbipunctata(威利斯顿，1893）是一种非食血昆虫，属于双翅目，亚目线虫（下双翅目）和科精神科。在目前的工作中,我们研究了C.albipunctata如何在不同的生理条件下控制它们的中肠pH，将它们的中肠生理与一些线虫食血物种进行比较。在摄入糖后测量C.albipunctata中肠pH值，蛋白质和在血粉后获得的嗜血沙蝇Lutzomialongialpis的血淋巴中释放的碱化激素的作用下。未饲喂或糖饲喂的C.albipunctata的中肠pH为5.5-6，其中肠在摄入蛋白质后或在用从血液饲喂的L.longipalpis收集的血淋巴处理下进行碱化。这些结果表明，在线虫中，pH控制机制似乎在嗜血和非嗜血物种之间共享。这种pH控制便于成功消化血液。来自下双翅目的许多食血组的独立进化表明，中肠pH控制所涉及的特征已经存在于非食血物种中，并且代表了适应这种喂养模式的准备。
