Abstract:
The aim of this study is to determine the roles of eNOS gene variations in BCA development. Our study included 91 patients diagnosed with BCA and 91 healthy controls. eNOS 4VNTR (4a/b), T786C and G894T gene variations genotype distributions were determined by PCR and RFLP methods. The significant difference was determined between these groups in terms of eNOS T786C and eNOS G894T gene variations genotype distributions (p < 0.05). TT genotype for G894T gene variation and CC genotype for T786C gene variation were detected higher in patients. The CC genotype of T786C gene variation was detected significantly higher in male patients than in male controls (p < 0.05). In addition, aa-TT, ab-TT, bb-TT haplotypes of 4VNTR (4a/b)-G894T gene variations, aa-CC, ab-CC, bb-CC haplotypes of 4VNTR (4a/b)-T786C gene variations and TT-TT, TT-CC, TT-CT, GG-CC, GT-CC haplotypes of G894T-T786C gene variations were observed in patient group more than control group. The significant difference was detected between these groups in terms of eNOS (G894T-T786C) haplotypes (p < 0.05). In our study, eNOS T786C and eNOS G894T gene variations were determined important genetic risk factor in the Thrace population of Turkey.
摘要:
本研究的目的是确定eNOS基因变异在BCA发育中的作用。我们的研究包括91例诊断为BCA的患者和91例健康对照。eNOS4VNTR(4a/b),通过PCR和RFLP方法确定T786C和G894T基因变异的基因型分布。在eNOST786C和eNOSG894T基因变异基因型分布方面,这些组之间存在显着差异(p<0.05)。TT基因型为G894T基因变异,CC基因型为T786C基因变异在患者中检出较高。在男性患者中检测到的T786C基因变异的CC基因型明显高于男性对照组(p<0.05)。此外,aa-TT,ab-TT,4VNTR(4a/b)-G894T基因变异的bb-TT单倍型,aa-CC,ab-CC,4VNTR(4a/b)-T786C基因变异的bb-CC单倍型和TT-TT,TT-CC,TT-CT,GG-CC,G894T-T786C基因的GT-CC单倍型变异在患者组较对照组多。在eNOS(G894T-T786C)单倍型方面,这些组之间检测到显着差异(p<0.05)。在我们的研究中,eNOST786C和eNOSG894T基因变异是确定土耳其色雷斯种群的重要遗传风险因子。