PDF(Pubmed)
Abstract：
UNASSIGNED: Bronchial asthma (BA) is a chronic inflammatory airway disease. Previous research has shown that Yanghe Pingchuan granules (YPG), among the granules formulated by the First Affiliated Hospital of the Anhui University of Chinese Medicine, exerts a precise therapeutic effect on BA. We previously showed that YPG improves airway inflammation in BA rats. Other studies have shown that the inhibitor of kappa-B kinase (IKK)/inhibitor of NF-κB (IκB)/nuclear factor kappa-B (NF-κB) signalling pathway plays a key role in inflammation mediation. Therefore, this study explored whether YPG could intervene in BA through the IKK/IκB/NF-κB signalling pathway.
UNASSIGNED: Ovalbumin-induced method was used to established BA rat model. After successful modelling, the authors used YPG to intervene the rats in BA rats. Hematoxylin-eosin (HE) staining was used to detect the bronchial pathological changes in BA rats, enzyme-linked immunosorbent assay (ELISA) was used to detect the changes of inflammatory factors (IL-1β and IL-6) and oxidative stress indexes malondialdehyde (MDA), superoxide dismutase (SOD) and nitrogen monoxide (NO), Quantitative real-time polymerase chain reactionCR and western blot were used to detect the expression of IKK/IκB/NF-κB signalling pathway.
UNASSIGNED: In BA model rats, YPG significantly improved the inflammatory response in bronchial tissues, reduced inflammatory factors IL-1β and IL-6, alleviated oxidative stress, reduced MDA and NO, and increased SOD. Quantitative real-time polymerase chain reaction and western blot results showed that YPG could block the IKK/IκB/NF-κB signalling pathway.
UNASSIGNED: These findings showed that YPG had a definite therapeutic effect on BA, which may be related to blocking the IKK/IκB/NF-κB signalling pathway and improving inflammation and oxidative stress.
UNASSIGNED: Ovalbumin-induced method was used to established BA rat model. After successful modelling, the authors used YPG to intervene the rats in BA rats. Hematoxylin-eosin (HE) staining was used to detect the bronchial pathological changes in BA rats, enzyme-linked immunosorbent assay (ELISA) was used to detect the changes of inflammatory factors (IL-1β and IL-6) and oxidative stress indexes malondialdehyde (MDA), superoxide dismutase (SOD) and nitrogen monoxide (NO), Quantitative real-time polymerase chain reactionCR and western blot were used to detect the expression of IKK/IκB/NF-κB signalling pathway.
UNASSIGNED: In BA model rats, YPG significantly improved the inflammatory response in bronchial tissues, reduced inflammatory factors IL-1β and IL-6, alleviated oxidative stress, reduced MDA and NO, and increased SOD. Quantitative real-time polymerase chain reaction and western blot results showed that YPG could block the IKK/IκB/NF-κB signalling pathway.
UNASSIGNED: These findings showed that YPG had a definite therapeutic effect on BA, which may be related to blocking the IKK/IκB/NF-κB signalling pathway and improving inflammation and oxidative stress.
摘要：
■支气管哮喘（BA）是一种慢性炎症性气道疾病。先前的研究表明，洋河平喘颗粒（YPG），在安徽中医药大学第一附属医院配制的颗粒中，对BA产生精确的治疗效果。我们先前显示YPG可改善BA大鼠的气道炎症。其他研究表明，κB激酶抑制剂（IKK）/NF-κB抑制剂（IκB）/核因子κB（NF-κB）信号通路在炎症介导中起关键作用。因此,这项研究探讨了YPG是否可以通过IKK/IκB/NF-κB信号通路干预BA。
■卵清蛋白诱导法建立BA大鼠模型。建模成功后,作者使用YPG对BA大鼠进行干预。苏木精-伊红(HE)染色检测BA大鼠支气管病理变化,酶联免疫吸附试验(ELISA)检测炎症因子(IL-1β、IL-6)和氧化应激指标丙二醛(MDA)的变化,超氧化物歧化酶（SOD）和一氧化氮（NO），实时定量聚合酶链反应CR和Westernblot检测IKK/IκB/NF-κB信号通路的表达。
■在BA模型大鼠中，YPG显著改善了支气管组织的炎症反应，降低炎症因子IL-1β和IL-6，减轻氧化应激，减少MDA和NO，增加SOD。定量实时聚合酶链反应和Westernblot结果显示YPG能够阻断IKK/IκB/NF-κB信号通路。
■这些发现表明YPG对BA有明确的治疗作用，这可能与阻断IKK/IκB/NF-κB信号通路、改善炎症和氧化应激有关。
■卵清蛋白诱导法建立BA大鼠模型。建模成功后,作者使用YPG对BA大鼠进行干预。苏木精-伊红(HE)染色检测BA大鼠支气管病理变化,酶联免疫吸附试验(ELISA)检测炎症因子(IL-1β、IL-6)和氧化应激指标丙二醛(MDA)的变化,超氧化物歧化酶（SOD）和一氧化氮（NO），实时定量聚合酶链反应CR和Westernblot检测IKK/IκB/NF-κB信号通路的表达。
■在BA模型大鼠中，YPG显著改善了支气管组织的炎症反应，降低炎症因子IL-1β和IL-6，减轻氧化应激，减少MDA和NO，增加SOD。定量实时聚合酶链反应和Westernblot结果显示YPG能够阻断IKK/IκB/NF-κB信号通路。
■这些发现表明YPG对BA有明确的治疗作用，这可能与阻断IKK/IκB/NF-κB信号通路、改善炎症和氧化应激有关。
