Abstract：
Pre-eclampsia is a serious multi-organ complication that severely threatens the safety of pregnant women and infants. To accurate and timely diagnose pre-eclampsia, point-of-care (POC) biosensing of the specific biomarkers is urgently required. However, one of the key biomarkers of pre-eclampsia, placental growth factor (PlGF), has a reduced level of expression in patients, which challenges the quantification capability and Limit-of-detection (LOD) of biosensors. Herein, we reported a microfiber Bragg grating biosensor for the quantification of PlGF in clinical serum samples. The Bragg grating was inscribed in a unilateral tapered fiber to generate the segmented Fabry-Perot spectrum for improving the capability of detection. Furthermore, a temperature-calibrated Bragg grating was added to enable dual parametric detection of PlGF and temperature simultaneously for removing the crosstalk. Finally, the biosensor was envisaged to be perfectly compatible with microfluidic chips, and thus dramatically reducing the sample consumption to as small as 10 μL. The proposed biosensor can respond to PlGF with concentrations ranging from 5 to 120 pg mL-1, attaining a LOD of 5 pg mL-1 of clinical relevance. More importantly, the biosensor achieved micro volume detection of clinical serum samples from patients, and the ROC curve with an AUC of 0.977 confirmed the viability of the device. Our study paves the way to a new idea for cost-effective and high-precision screening of patients with pre-eclampsia, and hence envisages a promising prospect for point-of-care (POC) diagnosis of patients with pre-eclampsia.
摘要：
子痫前期是一种严重的多器官并发症，严重威胁孕妇和婴儿的安全。为了准确及时地诊断子痫前期，迫切需要特定生物标志物的即时（POC）生物传感。然而,先兆子痫的关键生物标志物之一，胎盘生长因子(PlGF),患者的表达水平降低，这对生物传感器的定量能力和检测限(LOD)提出了挑战。在这里，我们报道了一种用于临床血清样本中PlGF定量的微光纤光栅生物传感器。将布拉格光栅刻写在单侧锥形光纤中，以生成分段的法布里-珀罗光谱，以提高检测能力。此外，添加了温度校准的布拉格光栅，以实现对PlGF和温度的双参数检测，以消除串扰。最后,生物传感器被设想与微流控芯片完全兼容，并因此将样品消耗显著降低至10μL。所提出的生物传感器可以响应浓度范围为5至120pgmL-1的PlGF，达到临床相关性的5pgmL-1的LOD。更重要的是,该生物传感器实现了对患者临床血清样本的微量检测，AUC为0.977的ROC曲线证实了该装置的可行性。我们的研究为先兆子痫患者的高性价比和高精度筛查铺平了道路。因此，为先兆子痫患者的即时护理（POC）诊断提供了有希望的前景。
