Abstract：
The ratio of β-1,4-glucosidase (BG) to acid/alkaline phosphomonoesterase (AP) (BG:AP) is commonly employed as an indicator to assess the relative microbial limitations of carbon (C) and phosphorus (P), whereby a higher BG:AP ratio suggests stronger C limitations. This approach is based on the assumption that BG and AP can represent enzymes targeting C and P, respectively. Nevertheless, it is crucial to recognize that microbial C and P acquisition involves the participation of other enzymes alongside BG and AP, and thus, the capacity of BG and AP to accurately and comprehensively represent the entire spectrum of C and P acquisition is questionable. Here, analyzing previously published data, I present a piece of empirical evidence that challenges the suitability of the BG:AP ratio as an accurate indicator of microbial limitations concerning C vs P. P fertilization decreased BG:AP in up to 27 % out of the total 109 observations, which represents a clear contradiction, as this outcome is interpreted by the enzymatic stoichiometry approach as indicating an intensified P limitation arising from P fertilization. Furthermore, the effect of P fertilization on the BG:AP ratio did not show significant differences between experimental sites characterized by higher BG:AP ratios (indicative of lesser P limitation) and those with lower BG:AP ratios (indicative of greater P limitation). Consequently, I conclude that the BG:AP ratio inadequately reflects microbial C vs P limitations.
摘要：
β-1,4-葡萄糖苷酶（BG）与酸/碱性磷酸单酯酶（AP）的比率（BG：AP）通常用作评估碳（C）和磷（P）的相对微生物限制的指标。由此,较高的BG:AP比率表明较强的C限制。这种方法基于BG和AP可以代表靶向C和P的酶的假设，分别。然而，至关重要的是要认识到微生物C和P的获得涉及其他酶与BG和AP的参与，因此，BG和AP准确和全面地表示C和P采集的整个频谱的能力是值得怀疑的。这里,分析以前发布的数据，IpresentapieceofexperimentalevidencethatchargesthesuitabilityoftheBG:APratioasanaccurateindicatorofmicrorantslimitationsconcerningCvsP.PfertilizationdecreasedBG:APinupto27%ofthetotal109observations,这代表了一个明显的矛盾，因为该结果被酶学化学计量方法解释为表明P施肥引起的P限制加剧。此外，P受精对BG:AP比值的影响在BG:AP比值较高(P限制较大)的实验地点和BG:AP比值较低(P限制较小)的实验地点之间没有显著差异.因此,我得出的结论是，BG：AP比率不足以反映微生物C与P的局限性。
