Abstract：
This study evaluated the effect of photobiomodulation therapy (PBMT) using 660 and 808 nm diode lasers (individual and in combination) on periodontal ligament mesenchymal stem cells (PDLSCs) in the presence of zoledronic acid (ZA). PDLSCs were cultured for 48 h in DMEM complete medium containing 5 μM ZA. PBMT was done three times with a 24-h interval in groups 1 (660 nm, 5 J/cm2 ), 2 (880 nm, 3 J/cm2 ), and 3 (660 + 808 nm) either in normal or ZA-treated culture medium. Control groups did not receive PBMT. Twenty-four hours post-irradiation, cell proliferation and expression of RANKL and OPG were assessed using MTT and real-time PCR tests, respectively. The results showed a significant decrease in cell viability in ZA-treated cells (p < 0.001). Additionally, ZA induced the expression of OPG (p = 0.03) while reducing RANKL (p < 0.001). Cell proliferation was significantly increased in 808 and 660 + 808 nm groups. Moreover, all PBMT groups could significantly increase and decrease the RANKL and OPG, respectively, in the presence of ZA (all p < 0.001). A combination of 660 + 808 nm showed the highest effects on both genes. In conclusion, it seems that PBMT can modulate the effects of ZA by inducing PDLSC proliferation and increasing RANKL-to-OPG gene expression ratio.
摘要：
这项研究评估了在唑来膦酸（ZA）存在下，使用660和808nm二极管激光（单独和组合）的光生物调节疗法（PBMT）对牙周膜间充质干细胞（PDLSC）的影响。将PDLSC在含有5μMZA的DMEM完全培养基中培养48小时。PBMT在第1组中进行了3次，间隔24小时（660nm，5J/cm2),2(880nm,3J/cm2),和3(660+808nm)在正常或ZA处理的培养基中。对照组不接受PBMT。照射后24小时，使用MTT和实时PCR测试评估RANKL和OPG的细胞增殖和表达，分别。结果显示ZA处理的细胞中细胞活力的显著降低(p<0.001)。此外,ZA诱导OPG的表达(p=0.03),同时降低RANKL(p<0.001)。808和660±808nm组细胞增殖显著增加。此外，所有PBMT组均能显著升高和降低RANKL和OPG，分别,在ZA的存在下（所有p<0.001）。660+808nm的组合显示对两个基因的最高影响。总之,PBMT似乎可以通过诱导PDLSC增殖和增加RANKL与OPG基因表达比率来调节ZA的作用。
