PDF(Pubmed)
Abstract：
BACKGROUND: The disease caused by Riemerella anatipestifer (R. anatipestifer, RA) results in large economic losses to the global duck industry every year. Serovar-related genomic variation, such as the O-antigen and capsular polysaccharide (CPS) gene clusters, has been widely used for serotyping in many gram-negative bacteria. RA has been classified into at least 21 serovars based on slide agglutination, but the molecular basis of serotyping is unknown. In this study, we performed a pan-genome-wide association study (Pan-GWAS) to identify the genetic loci associated with RA serovars.
RESULTS: The results revealed a significant association between the putative CPS synthesis gene locus and the serological phenotype. Further characterization of the CPS gene clusters in 11 representative serovar strains indicated that they were highly diverse and serovar-specific. The CPS gene cluster contained the key genes wzx and wzy, which are involved in the Wzx/Wzy-dependent pathway of CPS synthesis. Similar CPS loci have been found in some other species within the family Weeksellaceae. We have also shown that deletion of the wzy gene in RA results in capsular defects and cross-agglutination.
CONCLUSIONS: This study indicates that the CPS synthesis gene cluster of R. anatipestifer is a serotype-specific genetic locus. Importantly, our finding provides a new perspective for the systematic analysis of the genetic basis of the R anatipestifer serovars and a potential target for establishing a complete molecular serotyping scheme.
RESULTS: The results revealed a significant association between the putative CPS synthesis gene locus and the serological phenotype. Further characterization of the CPS gene clusters in 11 representative serovar strains indicated that they were highly diverse and serovar-specific. The CPS gene cluster contained the key genes wzx and wzy, which are involved in the Wzx/Wzy-dependent pathway of CPS synthesis. Similar CPS loci have been found in some other species within the family Weeksellaceae. We have also shown that deletion of the wzy gene in RA results in capsular defects and cross-agglutination.
CONCLUSIONS: This study indicates that the CPS synthesis gene cluster of R. anatipestifer is a serotype-specific genetic locus. Importantly, our finding provides a new perspective for the systematic analysis of the genetic basis of the R anatipestifer serovars and a potential target for establishing a complete molecular serotyping scheme.
摘要：
背景：由鼠疫Riemerella引起的疾病（R。anatipestifer,RA)每年都会给全球养鸭业带来巨大的经济损失。血清型相关基因组变异，如O-抗原和荚膜多糖(CPS)基因簇，已广泛用于许多革兰氏阴性菌的血清分型。根据载玻片凝集将RA分为至少21种血清型，但血清分型的分子基础尚不清楚.在这项研究中,我们进行了全基因组关联研究(Pan-GWAS),以鉴定与RA血清变型相关的遗传基因座.
结果：结果显示推定的CPS合成基因位点与血清学表型之间存在显著关联。11个代表性血清变型菌株中CPS基因簇的进一步表征表明，它们具有高度多样性和血清变型特异性。CPS基因簇包含关键基因wzx和wzy，参与CPS合成的Wzx/Wzy依赖性途径。在Weeksellaceae家族的其他一些物种中也发现了类似的CPS基因座。我们还表明，RA中wzy基因的缺失会导致荚膜缺陷和交叉凝集。
结论：本研究表明，鼠尾草的CPS合成基因簇是血清型特异性遗传位点。重要的是,我们的发现为系统分析Ranatipestifer血清变型的遗传基础提供了新的视角,也为建立完整的分子血清分型方案提供了潜在的靶点.
结果：结果显示推定的CPS合成基因位点与血清学表型之间存在显著关联。11个代表性血清变型菌株中CPS基因簇的进一步表征表明，它们具有高度多样性和血清变型特异性。CPS基因簇包含关键基因wzx和wzy，参与CPS合成的Wzx/Wzy依赖性途径。在Weeksellaceae家族的其他一些物种中也发现了类似的CPS基因座。我们还表明，RA中wzy基因的缺失会导致荚膜缺陷和交叉凝集。
结论：本研究表明，鼠尾草的CPS合成基因簇是血清型特异性遗传位点。重要的是,我们的发现为系统分析Ranatipestifer血清变型的遗传基础提供了新的视角,也为建立完整的分子血清分型方案提供了潜在的靶点.
