PDF(Pubmed)
Abstract：
This study aimed to investigate the impact of ambient lead (Pb) exposure on progesterone (P4) and pregnancy-associated glycoprotein 1 (PAG1) and their relationship with abortion in Egyptian Zaraibi goats (C. hircus). To achieve this, 40 female goats (does) were mated with highly fertile male goats, resulting in a total of 28 pregnant goats. Eight of them aborted, and each of the 12 pregnant goats gave birth to one kid, whereas the remaining eight gave birth to twins. The levels of PAG1, P4, and Pb in serum were estimated by enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and inductively coupled plasma mass spectrometry (ICP-MS) respectively. Statistically, the repeated measure two-way ANOVA, regression analysis, correlation coefficient, and receiver operating characteristic (ROC) curves were applied. The current data demonstrated that the levels of blood Pb in aborted goats were significantly higher than those in non-aborted goats at the early, mid, and late gestations, and this was followed by significant decreases in serum PAG1 and P4. Furthermore, there were substantial inverse associations between blood Pb concentration and levels of PAG1 and P4, with markedly negative correlation coefficients of - 0.88 and - 0.77, respectively, in aborted goats. The threshold level of Pb required to cause abortion was ≥ 32.08 μg/dl, but for PAG1 and P4 were respectively ≤ 0.95 ng/ml and ≤ 0.48 ng/ml. Additionally, threshold levels of ≥ 12.34 ng/ml and ≥ 31.52 ng/ml for P4 and PAG1, respectively, were needed to deliver twins. In conclusion, pollution-induced increases in Pb bioavailability resulted in dramatic decreases in P4 and PAG1 levels, leading to abortions. PAG1 and P4 levels are also key factors in determining whether Zaraibi goats will give birth to twins.
摘要：
本研究旨在研究环境铅（Pb）暴露对孕酮（P4）和妊娠相关糖蛋白1（PAG1）的影响及其与埃及扎莱比山羊流产的关系（C.hircus）。为了实现这一点，40只雌性山羊与高肥沃的雄性山羊交配，共有28只怀孕的山羊。其中八个流产了,12只怀孕的山羊每只都生了一个孩子，而剩下的八个生了双胞胎。采用酶联免疫吸附试验(ELISA)检测血清中PAG1、P4和Pb的水平,放射免疫分析（RIA），和电感耦合等离子体质谱(ICP-MS)。统计上,重复测量双向方差分析，回归分析，相关系数，并应用受试者工作特征(ROC)曲线。目前的数据表明，早期流产山羊的血铅水平明显高于非流产山羊，mid,和晚期妊娠，随后血清PAG1和P4显著下降。此外，血铅浓度与PAG1和P4水平呈显著负相关，相关系数分别为-0.88和-0.77。流产山羊。导致流产所需的铅阈值水平≥32.08μg/dl，但PAG1和P4分别≤0.95ng/ml和≤0.48ng/ml。此外,P4和PAG1的阈值水平分别为≥12.34ng/ml和≥31.52ng/ml，需要接生双胞胎。总之,污染引起的铅生物有效性增加导致P4和PAG1水平急剧下降，导致堕胎。PAG1和P4水平也是决定Zaraibi山羊是否会生双胞胎的关键因素。
