PDF(Pubmed)
Abstract：
UNASSIGNED: Tumor microenvironment is emerging as a critical factor for progression of breast cancer. Tumor-associated macrophages (TAMs) play an important role in promoting tumor growth.
UNASSIGNED: This study was aimed at correlation of number density (ND) of TAMs with invasive ductal carcinoma (IDC) grading utilizing an image morphometric technique. We also sought to compare the TAMs and ND in the tumoral area and stromal region. We also explored the relationship between the clinical and pathological prognostic parameters.
UNASSIGNED: The study included 75 cases of IDC that had undergone modified radical mastectomy. The Institutional Ethics Committee approved the study. Samples were classified as Grade 1, 2, and 3. Cases were graded as per the modified Bloom and Richardson criterion. Mean with standard deviation was calculated for each group. We utilized CD68 and CD163 immunostained sections for determining the ND of TAMs. TAMs were evaluated using computerized digital photomicrograph system with image analyzing software. ND was defined as the number of TAMs in total number of TAMs in five high-power fields/total area of five fields. ND was calculated separately in tumor and tumor stroma (TS). Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2/neu (HER2/neu) were scored in accordance with recommendations. Ki-67 was scored as per the recommended guidelines.
UNASSIGNED: Data were tabulated in Microsoft Excel. SPSS version 20.0 (IBM Corp., Armonk, NY, USA) was used for statistical analysis. To determine the relationship between macrophage density and clinicopathologic parameters, we used the independent t-test. To determine the differences in the parameters, analysis of variance (ANOVA) was utilized.
UNASSIGNED: Age of the patients ranged from 34 to 58 years (mean: 55.5). One-way ANOVA between various grades of tumor indicating significant differences in terms of CD68 and CD163 densities in tumor and stroma (P < 0.0001). i.e., significant increased density of CD68 and CD163 was observed in Grade 3 tumor as compared to other two groups. A greater histological grade, ER, PR negative status, and a high Ki-67 index were all associated with TAM ND. There was no relation to HER2/neu status. Result of unpaired t-test indicates increased density in stroma as compared to tumor among various grades of IDC.
UNASSIGNED: We analyzed images with a software using photographs of the stained slides. This helped in quantitative analysis of TAMs on the CD68 and CD163 stained sections. This approach standardizes and reproducibly counts TAMs per unit area. We found significant difference between the number densities of TAMs in grades of invasive breast carcinoma. There were statistically significant differences in numerical densities of TAMs with ER, PR negativity, and Ki-67. There was no correlation with HER2/neu. Densities of CD68 and CD163 densities are more prevalent in TS as compared to intratumoral region.
UNASSIGNED: This study was aimed at correlation of number density (ND) of TAMs with invasive ductal carcinoma (IDC) grading utilizing an image morphometric technique. We also sought to compare the TAMs and ND in the tumoral area and stromal region. We also explored the relationship between the clinical and pathological prognostic parameters.
UNASSIGNED: The study included 75 cases of IDC that had undergone modified radical mastectomy. The Institutional Ethics Committee approved the study. Samples were classified as Grade 1, 2, and 3. Cases were graded as per the modified Bloom and Richardson criterion. Mean with standard deviation was calculated for each group. We utilized CD68 and CD163 immunostained sections for determining the ND of TAMs. TAMs were evaluated using computerized digital photomicrograph system with image analyzing software. ND was defined as the number of TAMs in total number of TAMs in five high-power fields/total area of five fields. ND was calculated separately in tumor and tumor stroma (TS). Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2/neu (HER2/neu) were scored in accordance with recommendations. Ki-67 was scored as per the recommended guidelines.
UNASSIGNED: Data were tabulated in Microsoft Excel. SPSS version 20.0 (IBM Corp., Armonk, NY, USA) was used for statistical analysis. To determine the relationship between macrophage density and clinicopathologic parameters, we used the independent t-test. To determine the differences in the parameters, analysis of variance (ANOVA) was utilized.
UNASSIGNED: Age of the patients ranged from 34 to 58 years (mean: 55.5). One-way ANOVA between various grades of tumor indicating significant differences in terms of CD68 and CD163 densities in tumor and stroma (P < 0.0001). i.e., significant increased density of CD68 and CD163 was observed in Grade 3 tumor as compared to other two groups. A greater histological grade, ER, PR negative status, and a high Ki-67 index were all associated with TAM ND. There was no relation to HER2/neu status. Result of unpaired t-test indicates increased density in stroma as compared to tumor among various grades of IDC.
UNASSIGNED: We analyzed images with a software using photographs of the stained slides. This helped in quantitative analysis of TAMs on the CD68 and CD163 stained sections. This approach standardizes and reproducibly counts TAMs per unit area. We found significant difference between the number densities of TAMs in grades of invasive breast carcinoma. There were statistically significant differences in numerical densities of TAMs with ER, PR negativity, and Ki-67. There was no correlation with HER2/neu. Densities of CD68 and CD163 densities are more prevalent in TS as compared to intratumoral region.
摘要：
■肿瘤微环境正在成为乳腺癌进展的关键因素。肿瘤相关巨噬细胞(TAMs)在促进肿瘤生长中起重要作用。
■这项研究旨在利用图像形态计量学技术将TAM的数量密度（ND）与浸润性导管癌（IDC）分级相关联。我们还试图比较肿瘤区域和基质区域的TAM和ND。我们还探讨了临床和病理预后参数之间的关系。
■该研究包括75例接受改良根治术的IDC患者。机构伦理委员会批准了这项研究。样品被分类为1级、2级和3级。根据改良的Bloom和Richardson标准对病例进行分级。计算每组的平均值和标准偏差。我们使用CD68和CD163免疫染色切片确定TAM的ND。使用带有图像分析软件的计算机数字显微照相系统评估TAM。ND被定义为五个高功率场中的TAM总数/五个场的总面积中的TAM数。分别计算肿瘤和肿瘤基质（TS）中的ND。雌激素受体(ER),孕激素受体（PR），和人表皮生长因子受体2/neu（HER2/neu）按照建议进行评分。Ki-67按照推荐的指南进行评分。
■数据在MicrosoftExcel中列出。SPSS版本20.0(IBM公司,Armonk,NY,美国)用于统计分析。为了确定巨噬细胞密度与临床病理参数之间的关系，我们使用独立t检验。为了确定参数的差异，使用方差分析(ANOVA)。
■患者的年龄范围为34至58岁（平均：55.5）。不同级别肿瘤之间的单向ANOVA表明肿瘤和基质中CD68和CD163密度方面的显著差异(P<0.0001)。即，与其他两组相比，在3级肿瘤中观察到CD68和CD163的密度显着增加。组织学分级更高，ER,PR阴性状态，高Ki-67指数均与TAMND相关。与HER2/neu状态无关。非配对t检验的结果表明，与各种级别的IDC中的肿瘤相比，基质中的密度增加。
我们使用染色载玻片的照片用软件分析图像。这有助于CD68和CD163染色切片上的TAM的定量分析。这种方法标准化并可重复地对每单位面积的TAM进行计数。我们发现浸润性乳腺癌的TAM数量密度之间存在显着差异。TAM的数值密度与ER有统计学上的显着差异，公关的消极情绪，Ki-67与HER2/neu无相关性。与肿瘤内区域相比，TS中CD68和CD163密度的密度更普遍。
■这项研究旨在利用图像形态计量学技术将TAM的数量密度（ND）与浸润性导管癌（IDC）分级相关联。我们还试图比较肿瘤区域和基质区域的TAM和ND。我们还探讨了临床和病理预后参数之间的关系。
■该研究包括75例接受改良根治术的IDC患者。机构伦理委员会批准了这项研究。样品被分类为1级、2级和3级。根据改良的Bloom和Richardson标准对病例进行分级。计算每组的平均值和标准偏差。我们使用CD68和CD163免疫染色切片确定TAM的ND。使用带有图像分析软件的计算机数字显微照相系统评估TAM。ND被定义为五个高功率场中的TAM总数/五个场的总面积中的TAM数。分别计算肿瘤和肿瘤基质（TS）中的ND。雌激素受体(ER),孕激素受体（PR），和人表皮生长因子受体2/neu（HER2/neu）按照建议进行评分。Ki-67按照推荐的指南进行评分。
■数据在MicrosoftExcel中列出。SPSS版本20.0(IBM公司,Armonk,NY,美国)用于统计分析。为了确定巨噬细胞密度与临床病理参数之间的关系，我们使用独立t检验。为了确定参数的差异，使用方差分析(ANOVA)。
■患者的年龄范围为34至58岁（平均：55.5）。不同级别肿瘤之间的单向ANOVA表明肿瘤和基质中CD68和CD163密度方面的显著差异(P<0.0001)。即，与其他两组相比，在3级肿瘤中观察到CD68和CD163的密度显着增加。组织学分级更高，ER,PR阴性状态，高Ki-67指数均与TAMND相关。与HER2/neu状态无关。非配对t检验的结果表明，与各种级别的IDC中的肿瘤相比，基质中的密度增加。
我们使用染色载玻片的照片用软件分析图像。这有助于CD68和CD163染色切片上的TAM的定量分析。这种方法标准化并可重复地对每单位面积的TAM进行计数。我们发现浸润性乳腺癌的TAM数量密度之间存在显着差异。TAM的数值密度与ER有统计学上的显着差异，公关的消极情绪，Ki-67与HER2/neu无相关性。与肿瘤内区域相比，TS中CD68和CD163密度的密度更普遍。
