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Abstract：
UNASSIGNED: Bladder carcinoma ranks tenth among all cancers worldwide predominantly affecting elderly males. Common risk factors being cigarette smoke and aniline dyes. Immunohistochemical markers play a pivotal role for its diagnosis and prognosis.
UNASSIGNED: To analyze the immunohistochemical expression of p53, CD10, Ki-67 in bladder cancers correlating with demographic features, pathological grade, and stage and to establish as prognostic biomarkers.
UNASSIGNED: Surgical samples of total of 70 cases of bladder tumor were collected, processed, stained in routine hematoxylin and eosin followed by immunohistochemistry of p53, CD10, and Ki67 markers performed on randomly selected 30 cases only.
UNASSIGNED: Out of 70 cases 69 cases (98.6%) were carcinomas; urothelial carcinoma being 71.4% (n = 50) with male: female ratio = 7.7:1 and mean age = 61.81 ± 12.83 years. Out of 30 cases, p53 was positive in 50% of cases, 30% - negative and 20% - equivocal. p53 positive expression pattern was more in high grade (HG) than low grade (LG). Significant difference was observed in the mean p53 scoring (%) and different stages (P = 0.043). CD10 expression was negative in 56.6%, (1+) in 16.6%, and (2+) in 26.6% of cases and significant difference in CD 10 expression was observed between the high and LG (P = 0.001). Ki-67 labeling index was appreciably higher in HG than the LG tumor (32.49% ± 24.35%; 6.86% ± 8.1%). Majority of Ki-67 expression was observed in stage pT2, followed by the pT1 stage.
UNASSIGNED: Cocktail of p53, CD10, and Ki67 is useful as potential prognostic markers in bladder cancers.
UNASSIGNED: To analyze the immunohistochemical expression of p53, CD10, Ki-67 in bladder cancers correlating with demographic features, pathological grade, and stage and to establish as prognostic biomarkers.
UNASSIGNED: Surgical samples of total of 70 cases of bladder tumor were collected, processed, stained in routine hematoxylin and eosin followed by immunohistochemistry of p53, CD10, and Ki67 markers performed on randomly selected 30 cases only.
UNASSIGNED: Out of 70 cases 69 cases (98.6%) were carcinomas; urothelial carcinoma being 71.4% (n = 50) with male: female ratio = 7.7:1 and mean age = 61.81 ± 12.83 years. Out of 30 cases, p53 was positive in 50% of cases, 30% - negative and 20% - equivocal. p53 positive expression pattern was more in high grade (HG) than low grade (LG). Significant difference was observed in the mean p53 scoring (%) and different stages (P = 0.043). CD10 expression was negative in 56.6%, (1+) in 16.6%, and (2+) in 26.6% of cases and significant difference in CD 10 expression was observed between the high and LG (P = 0.001). Ki-67 labeling index was appreciably higher in HG than the LG tumor (32.49% ± 24.35%; 6.86% ± 8.1%). Majority of Ki-67 expression was observed in stage pT2, followed by the pT1 stage.
UNASSIGNED: Cocktail of p53, CD10, and Ki67 is useful as potential prognostic markers in bladder cancers.
摘要：
■膀胱癌在全球所有癌症中排名第十，主要影响老年男性。常见的危险因素是香烟烟雾和苯胺染料。免疫组织化学标志物在其诊断和预后中起着重要作用。
■分析膀胱癌中p53，CD10，Ki-67的免疫组织化学表达与人口学特征相关，病理分级,和分期，并建立为预后生物标志物。
■共收集70例膀胱肿瘤的手术标本，已处理,在常规苏木精和伊红中染色，然后仅在随机选择的30例病例中进行p53，CD10和Ki67标记的免疫组织化学。
■70例中69例（98.6%）为癌；尿路上皮癌为71.4%（n=50），男女比例为7.7:1，平均年龄为61.81±12.83岁。在30个案例中，在50%的病例中p53为阳性,30%-阴性和20%-模棱两可。p53阳性表达模式在高级别（HG）中比在低级别（LG）中更多。在平均p53评分（％）和不同阶段（P=0.043）中观察到显着差异。56.6%的CD10表达为阴性,（1+）占16.6%，在26.6％的病例中（2），在high和LG之间观察到CD10表达的显着差异（P=0.001）。HG中的Ki-67标记指数明显高于LG肿瘤（32.49％±24.35％；6.86％±8.1％）。在pT2阶段观察到大多数Ki-67表达，其次是pT1阶段。
■p53、CD10和Ki67的混合物可用作膀胱癌的潜在预后标志物。
■分析膀胱癌中p53，CD10，Ki-67的免疫组织化学表达与人口学特征相关，病理分级,和分期，并建立为预后生物标志物。
■共收集70例膀胱肿瘤的手术标本，已处理,在常规苏木精和伊红中染色，然后仅在随机选择的30例病例中进行p53，CD10和Ki67标记的免疫组织化学。
■70例中69例（98.6%）为癌；尿路上皮癌为71.4%（n=50），男女比例为7.7:1，平均年龄为61.81±12.83岁。在30个案例中，在50%的病例中p53为阳性,30%-阴性和20%-模棱两可。p53阳性表达模式在高级别（HG）中比在低级别（LG）中更多。在平均p53评分（％）和不同阶段（P=0.043）中观察到显着差异。56.6%的CD10表达为阴性,（1+）占16.6%，在26.6％的病例中（2），在high和LG之间观察到CD10表达的显着差异（P=0.001）。HG中的Ki-67标记指数明显高于LG肿瘤（32.49％±24.35％；6.86％±8.1％）。在pT2阶段观察到大多数Ki-67表达，其次是pT1阶段。
■p53、CD10和Ki67的混合物可用作膀胱癌的潜在预后标志物。
