Abstract：
Coronavirus disease 2019 is a disease caused by the novel severe acute respiratory syndrome coronavirus 2. The double-positive of angiotensin-converting enzyme 2 and transmembrane protease serine type 2 have a higher risk of being infected by severe acute respiratory syndrome coronavirus 2. The susceptibility of coronavirus disease 2019 in patients with chronic diseases, especially in different tissues of ocular hypertension eyes like glaucoma, is not yet known.
An ocular hypertension model was established by laser photocoagulation in rhesus monkeys. The expression of angiotensin-converting enzyme 2 and transmembrane protease serine type 2 in three ocular hypertension eyes and the three control eyes were analyzed using immunofluorescence.
No difference was observed between ocular hypertension and control eyes in the expression of angiotensin-converting enzyme 2 and transmembrane protease serine type 2 in the conjunctival epithelium, corneal epithelium, and ciliary muscle. In ocular hypertension eyes and control eyes, angiotensin-converting enzyme 2 and transmembrane protease serine type 2 expression were both observed in the retina. Angiotensin-converting enzyme 2 staining of retinal ganglion cells was found to be significantly higher in ocular hypertension eyes than in control eyes. However, there was no difference in angiotensin-converting enzyme 2 and transmembrane protease serine type 2 expression in retinal vessels and choroidal vessels between ocular hypertension and control eyes. In our study, the expression and distribution of angiotensin-converting enzyme 2 and TMPREE2 in human retina were similar to that of non-human primates as expected.
Our study confirmed that angiotensin-converting enzyme 2 and transmembrane protease serine type 2 were expressed widely in rhesus monkey eyes. When compared with controls eyes, the expression of angiotensin-converting enzyme 2 was higher in the retinal ganglion cells in ocular hypertension eyes, suggesting that high ocular pressure may affect the patients\' ocular susceptibility to severe acute respiratory syndrome coronavirus 2 infection.
An ocular hypertension model was established by laser photocoagulation in rhesus monkeys. The expression of angiotensin-converting enzyme 2 and transmembrane protease serine type 2 in three ocular hypertension eyes and the three control eyes were analyzed using immunofluorescence.
No difference was observed between ocular hypertension and control eyes in the expression of angiotensin-converting enzyme 2 and transmembrane protease serine type 2 in the conjunctival epithelium, corneal epithelium, and ciliary muscle. In ocular hypertension eyes and control eyes, angiotensin-converting enzyme 2 and transmembrane protease serine type 2 expression were both observed in the retina. Angiotensin-converting enzyme 2 staining of retinal ganglion cells was found to be significantly higher in ocular hypertension eyes than in control eyes. However, there was no difference in angiotensin-converting enzyme 2 and transmembrane protease serine type 2 expression in retinal vessels and choroidal vessels between ocular hypertension and control eyes. In our study, the expression and distribution of angiotensin-converting enzyme 2 and TMPREE2 in human retina were similar to that of non-human primates as expected.
Our study confirmed that angiotensin-converting enzyme 2 and transmembrane protease serine type 2 were expressed widely in rhesus monkey eyes. When compared with controls eyes, the expression of angiotensin-converting enzyme 2 was higher in the retinal ganglion cells in ocular hypertension eyes, suggesting that high ocular pressure may affect the patients\' ocular susceptibility to severe acute respiratory syndrome coronavirus 2 infection.
摘要：
■2019年冠状病毒病是一种由新型严重急性呼吸道综合症冠状病毒2引起的疾病。血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型双阳性的人感染严重急性呼吸综合征冠状病毒2的风险较高。慢性病患者2019年冠状病毒病的易感性，特别是在高眼压的不同组织中，如青光眼，还不知道。
■通过恒河猴的激光光凝法建立了高眼压模型。采用免疫荧光法分析血管紧张素转换酶2和跨膜丝氨酸蛋白酶2在三只高眼及三只对照眼中的表达。
■高眼压和对照组眼在结膜上皮中血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型的表达没有差异，角膜上皮,和睫状肌.在高眼压眼和控制眼，血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型均在视网膜中表达。发现高眼压眼的视网膜神经节细胞的血管紧张素转换酶2染色明显高于对照组。然而,高眼压眼和对照眼的视网膜血管和脉络膜血管中血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型表达无差异。在我们的研究中,血管紧张素转换酶2和TMPREE2在人视网膜中的表达和分布与预期的非人灵长类动物相似。
■我们的研究证实，血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型在恒河猴眼中广泛表达。与对照组的眼睛相比，血管紧张素转换酶2在高眼压眼视网膜神经节细胞中的表达较高，提示高眼压可能影响患者眼部对严重急性呼吸道综合征冠状病毒2感染的易感性。
■通过恒河猴的激光光凝法建立了高眼压模型。采用免疫荧光法分析血管紧张素转换酶2和跨膜丝氨酸蛋白酶2在三只高眼及三只对照眼中的表达。
■高眼压和对照组眼在结膜上皮中血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型的表达没有差异，角膜上皮,和睫状肌.在高眼压眼和控制眼，血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型均在视网膜中表达。发现高眼压眼的视网膜神经节细胞的血管紧张素转换酶2染色明显高于对照组。然而,高眼压眼和对照眼的视网膜血管和脉络膜血管中血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型表达无差异。在我们的研究中,血管紧张素转换酶2和TMPREE2在人视网膜中的表达和分布与预期的非人灵长类动物相似。
■我们的研究证实，血管紧张素转换酶2和跨膜蛋白酶丝氨酸2型在恒河猴眼中广泛表达。与对照组的眼睛相比，血管紧张素转换酶2在高眼压眼视网膜神经节细胞中的表达较高，提示高眼压可能影响患者眼部对严重急性呼吸道综合征冠状病毒2感染的易感性。
