Abstract：
OBJECTIVE: Polypropylene meshes (PM) used in pelvic organ prolapse surgery are being withdrawn from the market. Although concerns about the usage of PMs in stress incontinence surgery have been raised, it is still one of the best methods of curing stress urinary incontinence. With advancements in stem cell-based therapies, especially mesenchymal stem cells (MSCs), it is believed that coating the synthetic meshes with MSCs may minimize excessive tissue reactions ultimately leading to clinical problems such as pain, erosion or extrusion of the implanted material. In our study we tried to show the possibility of coating the PM with placenta-derived MSCs.
METHODS: Mesenchymal stem cells obtained from six placentas were isolated, cultured, and identified. MSCs were then soaked in either fibronectin or collagen prior to co-culturing with strips of PMs. One group is used as a control, and hence was not pretreated before co-culturing. Specimens were fixed and stained with both Gram and hematoxylin and eosin and marked with Vybran Dil and DAPI. All preparations were examined under a light microscope. The IMAGEJ program was utilized to determine the surface area of meshes coated with MSCs.
RESULTS: We clearly showed that PMs can be coated successfully with placenta-derived MSCs. The percentage of the coated area is significantly increased when meshes were pretreated with fibronectin or collagen (p<0.0001).
CONCLUSIONS: Placenta-derived MSCs can successfully coat PMs. The immunomodulatory properties of MSCs, which may be of great advantage in preventing the side effects of meshes, should be tested by in vivo and hopefully human studies before clinical applications.
METHODS: Mesenchymal stem cells obtained from six placentas were isolated, cultured, and identified. MSCs were then soaked in either fibronectin or collagen prior to co-culturing with strips of PMs. One group is used as a control, and hence was not pretreated before co-culturing. Specimens were fixed and stained with both Gram and hematoxylin and eosin and marked with Vybran Dil and DAPI. All preparations were examined under a light microscope. The IMAGEJ program was utilized to determine the surface area of meshes coated with MSCs.
RESULTS: We clearly showed that PMs can be coated successfully with placenta-derived MSCs. The percentage of the coated area is significantly increased when meshes were pretreated with fibronectin or collagen (p<0.0001).
CONCLUSIONS: Placenta-derived MSCs can successfully coat PMs. The immunomodulatory properties of MSCs, which may be of great advantage in preventing the side effects of meshes, should be tested by in vivo and hopefully human studies before clinical applications.
摘要：
目的：用于盆腔器官脱垂手术的聚丙烯网（PM）正在退出市场。尽管人们对PM在压力性尿失禁手术中的使用感到担忧，它仍然是治疗压力性尿失禁的最佳方法之一。随着干细胞疗法的进步，特别是间充质干细胞（MSCs），据认为，用MSC包被合成网状物可以最大限度地减少过度的组织反应，最终导致临床问题，如疼痛，植入材料的侵蚀或挤压。在我们的研究中，我们试图证明用胎盘来源的MSCs包被PM的可能性。
方法：分离6个胎盘的间充质干细胞，培养,并确定。然后将MSC浸泡在纤连蛋白或胶原蛋白中，然后与PMs条共培养。一组用作对照，因此在共培养之前没有预处理。将样品固定并用革兰氏和苏木精和曙红染色,并用VybranDil和DAPI标记。在光学显微镜下检查所有制剂。利用IMAGEJ程序来确定用MSC涂覆的网格的表面积。
结果：我们清楚地表明，PMs可以成功地用胎盘来源的MSCs包被。当用纤连蛋白或胶原预处理网状物时,涂覆面积的百分比显著增加(p<0.0001)。
结论：胎盘来源的MSCs可以成功包被PMs。这在防止网格的副作用方面可能有很大的优势，在临床应用之前，应该通过体内和希望的人体研究进行测试。
方法：分离6个胎盘的间充质干细胞，培养,并确定。然后将MSC浸泡在纤连蛋白或胶原蛋白中，然后与PMs条共培养。一组用作对照，因此在共培养之前没有预处理。将样品固定并用革兰氏和苏木精和曙红染色,并用VybranDil和DAPI标记。在光学显微镜下检查所有制剂。利用IMAGEJ程序来确定用MSC涂覆的网格的表面积。
结果：我们清楚地表明，PMs可以成功地用胎盘来源的MSCs包被。当用纤连蛋白或胶原预处理网状物时,涂覆面积的百分比显著增加(p<0.0001)。
结论：胎盘来源的MSCs可以成功包被PMs。这在防止网格的副作用方面可能有很大的优势，在临床应用之前，应该通过体内和希望的人体研究进行测试。
