PDF(Pubmed)
Abstract：
Colocasia esculenta (L.) Schott is a tuberous plant, also known as taro, employed as food worldwide for its renowned nutritional properties but also traditionally used in several countries for medical purposes. In this study, methanolic extracts were prepared from the corms and leaves of Colocasia, subsequently fractionated via molecular exclusion chromatography (RP-HPLC) and their anti-tumor activity assessed in an in vitro model of gastric adenocarcinoma (AGS cells). Vorm extract and isolated fractions II and III affected AGS cell vitality in a dose-dependent manner through the modulation of key proteins involved in cell proliferation, apoptosis, and cell cycle processes, such as caspase 3, cyclin A, cdk2, IkBα, and ERK. To identify bioactive molecules responsible for anti-tumoral activity fractions II and III were further purified via RP-HPLC and characterized via nuclear magnetic resonance (NMR) and electrospray mass spectrometry (ESI-MS) techniques. The procedure enabled the identification of ten compounds including lignans and neolignans, some isolated for the first time in taro, uncommon megastigmane derivatives, and a gallic acid derivative. However, none of the isolated constituents showed efficacy equivalent to that of the fractions and total extract. This suggests that the whole Colocasia phytocomplex has intriguing anti-tumor activity against gastric cancer.
摘要：
科罗卡西亚（L.）肖特是一种块茎植物，也被称为芋，因其著名的营养特性而在全球范围内用作食品，但传统上也在几个国家用于医疗目的。在这项研究中,甲醇提取物是从哥伦比亚的球茎和叶子中制备的，随后通过分子排阻层析（RP-HPLC）进行分级，并在胃腺癌（AGS细胞）的体外模型中评估其抗肿瘤活性。Vorm提取物和分离的组分II和III通过调节参与细胞增殖的关键蛋白以剂量依赖的方式影响AGS细胞活力，凋亡，和细胞周期过程，如胱天蛋白酶3，细胞周期蛋白A，cdk2,Ikbα,和ERK.为了鉴定负责抗肿瘤活性的生物活性分子,通过RP-HPLC进一步纯化级分II和III,并通过核磁共振(NMR)和电喷雾质谱(ESI-MS)技术表征。该程序使十种化合物的鉴定，包括木脂素和新木脂素，有些人第一次在芋里被隔离，不常见的megastigmane衍生物，和没食子酸衍生物。然而,没有一种分离的成分显示出与部分和总提取物相当的功效。这表明整个Colocasia植物复合物对胃癌具有有趣的抗肿瘤活性。
