PDF(Pubmed)
Abstract：
Owing to increasing air pollution due to industrial development, fine dust has been associated with threatening public health. In particular, ultrafine urban particulate matter (uf-UP, PM 0.1) can easily enter our bodies, causing inflammation-related diseases. Therefore, in the present study, we evaluated the effects of hydrothermal extracts of Sargassum horneri and its bioactive compound, loliolide, on uf-UP-induced inflammation as a potential treatment strategy for retinal disorders. Human retinal pigment epithelial cells (ARPE-19) stimulated with TNF-α or uf-UPs were treated with S. horneri extract and loliolide. S. horneri extracts exhibited anti-inflammatory effects on uf-UP-induced inflammation without cell toxicity through downregulating the mRNA expression of MCP-1, IL-8, IL-6, and TNF-α. UPLC-QTOF/MS analysis confirmed that the hydrothermal extract of S. horneri contained loliolide, which has anti-inflammatory effects. Loliolide effectively reduced the mRNA expression and production of proinflammatory chemokines (IL-8) and cytokines (IL-1β and IL-6) by downregulating the MAPK/NF-ĸB signaling pathway on TNF-α-stimulated inflammatory ARPE-19 cells. These effects were further confirmed in inflammatory ARPE-19 cells after stimulation with uf-UPs. Collectively, these results suggested the application of S. horneri as a functional ingredient for treating ocular disorders caused by particular matters.
摘要：
由于工业发展导致空气污染加剧，细小的粉尘与威胁公众健康有关。特别是,超细城市颗粒物(uf-up，PM0.1)可以很容易地进入我们的身体，引起炎症相关疾病。因此,在本研究中，我们评估了马尾藻及其生物活性化合物的水热提取物的效果，洛利内酯,uf-UP诱导的炎症作为视网膜疾病的潜在治疗策略。用horneri提取物和loliolide处理用TNF-α或uf-UPs刺激的人视网膜色素上皮细胞（ARPE-19）。通过下调MCP-1，IL-8，IL-6和TNF-α的mRNA表达，horneri提取物对uf-UP诱导的炎症表现出抗炎作用，而无细胞毒性。UPLC-QTOF/MS分析证实，霍氏链球菌的水热提取物含有洛利内酯，具有抗炎作用。Loliolide通过下调TNF-α刺激的炎性ARPE-19细胞的MAPK/NF-κB信号通路，有效降低了促炎趋化因子（IL-8）和细胞因子（IL-1β和IL-6）的mRNA表达和产生。在用uf-UPs刺激后,在炎性ARPE-19细胞中进一步证实了这些作用。总的来说，这些结果表明horneri作为功能性成分用于治疗特定物质引起的眼部疾病。
