PDF(Pubmed)
Abstract：
Structurally well-defined compounds have advantages for quality control in plant biostimulant production and application processes. Humic acid (HA) is a biostimulant that significantly affects plant growth, and soil-dwelling Protaetia brevitarsis larva (PBLs) can rapidly convert agricultural waste into HA. In this study, we use PBLs as a model to investigate HA formation and screen for structurally well-defined HA-related plant biostimulant compounds. Dephasing magic angle spinning nuclear magnetic resonance (13C DD-MAS NMR) analysis indicated HA structural changes during PBL digestion; metabolic profiling detected seven HA-related aromatic ring-containing compounds. A total of six compounds that significantly stimulate plant growth were identified through plant experiments, and all six compounds demonstrate the ability to enhance seed germination. It is noteworthy that piperic acid exhibits a remarkable promotion of root growth in plants, a finding reported for the first time in this study. Thus, this study not only provides insights into the insect-mediated transformation of HA but also illustrates a new method for discovering structurally well-defined plant biostimulant compounds.
摘要：
结构明确的化合物对于植物生物刺激剂生产和应用过程中的质量控制具有优势。腐植酸（HA）是一种生物刺激剂，显着影响植物生长，和居住在土壤中的ProtaetiaBrevitarsis幼虫（PBLs）可以将农业废物迅速转化为HA。在这项研究中,我们使用PBLs作为模型来研究HA形成并筛选结构明确的HA相关植物生物刺激剂化合物。去相魔角旋转核磁共振（13CDD-MASNMR）分析表明PBL消化过程中HA结构发生变化；代谢谱分析检测到七个与HA相关的含芳香环化合物。通过植物实验共鉴定出6种显著刺激植物生长的化合物,所有六种化合物都显示出增强种子萌发的能力。值得注意的是，胡椒酸在植物中表现出显着的促进根生长，这项研究首次报道了这一发现。因此，这项研究不仅提供了对昆虫介导的HA转化的见解，而且还说明了一种发现结构明确的植物生物刺激剂化合物的新方法。
