PDF(Pubmed)
Abstract：
Punica granatum L., commonly known as the pomegranate, is an abundant source of polyphenols, including hydrolyzable ellagitannins, ellagic acid, anthocyanins, and other bioactive phytochemicals shown to be effective in defending against oxidative stress, and has immunomodulatory activities. Ellagitannins, and their hydrolyzed product ellagic acid, interact with the gut microbiota to yield secondary metabolites known as urolithins that may have health benefits. The objective of this study was to determine the effects of supplementation with a standardized punicalagin-enriched pomegranate extract, Pomella® (250 mg), on the gut microbiome, circulating short-chain fatty acids, and gut microbial-derived ellagitannin metabolite urolithins. A randomized, double-blind, placebo-controlled study was conducted over 4 weeks on healthy volunteers aged 25-55 years. Subjects were randomly assigned to receive either an oral supplement containing 75 mg of punicalagin or an oral placebo. Stool sample collection and venipuncture were performed to analyze the gut microbiome, SCFAs, and urolithin. There was no significant change in the gut microbial diversity in both cohorts after 4 weeks of intervention, but there was a significantly increased relative abundance of Coprococcus eutectus, Roseburia faecis, Roseburia inullnivorans, Ruminococcus bicirculans, Ruminococcus calidus, and Faecalibacterium prausnitzii. Pomegranate extract (PE) supplementation led to the augmentation of circulating propionate levels (p = 0.02) and an increasing trend for acetate levels (p = 0.12). The pomegranate extract (PE) supplementation group had an increased level of circulating urolithins compared to the placebo group (6.6% vs. 1.1%, p = 0.13). PE supplementation correlated with shifts in the gut microbiome and with higher circulating levels of propionate and acetate. Further studies should explore the implications in larger cohorts and over a longer duration.
摘要：
石榴，俗称石榴，是多酚的丰富来源，包括可水解的鞣剂,鞣花酸,花青素,和其他生物活性植物化学物质显示出有效的防御氧化应激，并具有免疫调节活性。Ellagitannins,和它们的水解产物鞣花酸，与肠道微生物群相互作用，产生次生代谢产物，称为尿石素，可能对健康有益。这项研究的目的是确定补充标准的富含panicalagin的石榴提取物的效果，Pomella®（250毫克），在肠道微生物组中，循环短链脂肪酸，和肠道微生物来源的鞣花宁代谢产物尿石素。一个随机的，双盲,安慰剂对照研究在4周内对25-55岁的健康志愿者进行.受试者被随机分配接受含有75mgpunicalagin的口服补充剂或口服安慰剂。进行粪便样本收集和静脉穿刺以分析肠道微生物组，SCFA,和尿石素.干预4周后，两组的肠道微生物多样性均无显著变化，但是共析球菌的相对丰度显著增加，Roseburiafaecis,inullnivoransRoseburiainullnivorans,双歧球菌,杯状罗米球菌,和prausnitzii粪杆菌.石榴提取物（PE）的补充导致循环丙酸水平的增加（p=0.02）和乙酸水平的增加趋势（p=0.12）。与安慰剂组相比，石榴提取物（PE）补充组的循环尿石素水平增加（6.6％vs.1.1%,p=0.13）。PE补充与肠道微生物组的变化以及丙酸盐和乙酸盐的较高循环水平相关。进一步的研究应该探索在更大的队列和更长的持续时间的影响。
