PDF(Pubmed)
Abstract：
Coccidiosis poses a significant challenge in poultry production and is typically managed with ionophores and chemical anticoccidials. However, the emergence of drug resistance and limitations on their use have encouraged the exploration of alternative solutions, including botanical compounds and improvements in in vitro screening methods. Prior research focused only on the impact of these alternatives on Eimeria invasion, with intracellular development in cell cultures receiving limited attention. This study assessed the impact of thyme (Thymus vulgaris), oregano (Origanum vulgare), and garlic (Allium sativum) essential oils, as well as their bioactive compounds, on the initial phase of schizogony in Madin-Darby bovine kidney cells, comparing their effectiveness to two commercially used anticoccidial drugs. Using image analysis and quantitative PCR, the study confirmed the efficacy of commercial anticoccidials in reducing invasion and schizont formation, and it found that essential oils were equally effective. Notably, thymol and carvacrol exhibited mild inhibition of intracellular replication of the parasite but significantly reduced schizont numbers, implying a potential reduction in pathogenicity. In conclusion, this research highlights the promise of essential oils and their bioactive components as viable alternatives to traditional anticoccidial drugs for mitigating coccidiosis in poultry, particularly by disrupting the intracellular development of the parasites.
摘要：
球虫病对家禽生产构成重大挑战，通常使用离子载体和化学抗球虫药进行管理。然而,耐药性的出现和对其使用的限制鼓励了对替代解决方案的探索，包括植物化合物和体外筛选方法的改进。先前的研究仅集中在这些替代品对艾美球虫入侵的影响上，细胞培养物中的细胞内发育受到的关注有限。这项研究评估了百里香（Thymusvulgaris）的影响，牛至(Ohoganumvulgare),和大蒜精油，以及它们的生物活性化合物，在Madin-Darby牛肾细胞分裂的初始阶段，将其有效性与两种商业使用的抗球虫药物进行比较。使用图像分析和定量PCR，该研究证实了商业抗球虫药物在减少入侵和裂殖形成方面的功效，发现精油同样有效。值得注意的是,百里酚和香芹酚对寄生虫的细胞内复制表现出温和的抑制作用，但显着减少了裂殖体数，意味着致病性的潜在降低。总之,这项研究强调了精油及其生物活性成分作为传统抗球虫药物的可行替代品的前景，可以减轻家禽中的球虫病，特别是通过破坏寄生虫的细胞内发育。
