Abstract：
Micropterus salmoides rhabdovirus (MSRV) is one of the main pathogens of largemouth bass, leading to serious economic losses. The G protein, as the only envelope protein present on the surface of MSRV virion, contains immune-related antigenic determinants, thereby becoming the primary target for the design of MSRV vaccines. Here, we displayed the G protein on the surface of yeast cells (named EBY100/pYD1-G) and conducted a preliminary assessment of the protective efficacy of the recombinant yeast vaccine. Upon oral vaccination, a robust immune response was observed in systemic and mucosal tissue. Remarkably, following the MSRV challenge, the relative percent survival of EBY100/pYD1-G treated largemouth bass significantly increased to 66.7 %. In addition, oral administration inhibited viral replication and alleviated the pathological symptoms of MSRV-infected largemouth bass. These results suggest that EBY100/pYD1-G could be used as a potential oral vaccine against MSRV infection.
摘要：
小翅目salmoides弹状病毒(MSRV)是大嘴鲈鱼的主要病原之一,造成严重的经济损失。G蛋白，作为MSRV病毒粒子表面上唯一的包膜蛋白，含有免疫相关的抗原决定簇，从而成为设计MSRV疫苗的主要目标。这里,我们在酵母细胞表面展示了G蛋白（命名为EBY100/pYD1-G），并对重组酵母疫苗的保护效果进行了初步评估。口服疫苗后，在全身和粘膜组织中观察到强烈的免疫反应.值得注意的是,在MSRV挑战之后，EBY100/pYD1-G治疗的大口鲈鱼的相对存活率显着提高至66.7％。此外,口服给药抑制病毒复制并减轻MSRV感染的大口鲈鱼的病理症状。这些结果表明EBY100/pYD1-G可用作抗MSRV感染的潜在口服疫苗。
