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Abstract：
This study aimed to genotype isolates of Toxoplasma gondii obtained from samples of brain, diaphragm and heart of goats and sheep intended for human consumption in the State of Paraíba, Brazil. Tissue samples from 14 animals, goats (n = 5) and lambs (n = 9), were sourced from public slaughterhouses in seven cities and bio-assayed in mice. The brains of the mice were utilized for DNA extraction. Genotyping was carried out by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using 10 markers (SAG1, SAG2, SAG3, BTUB, c22-8, PK1, GRA6, L358, c-29-2 and Apico). A total of 10 isolates were fully genotyped (i.e. at all loci), three from goats and seven from sheep, revealing five distinct genotypes: #13 (n = 4); #48 (n = 3); #57 (n = 1); #273 (n = 1); and one new genotype that had not been previously described. Genotype #13 is frequently found in the Northeast of Brazil and represents a clonal lineage circulating in this region and was the most prevalent genotype identified (n = 4). Moreover, in the present study genotypes #13, #48, #57, and #273 were documented for the first time in sheep from Brazil, and the novel genotype was isolated from a goat. Our findings align with previous studies on T. gondii from Brazil, where new genotypes are continuously being identified, highlighting a high level of genetic diversity of T. gondii isolates in the country.
摘要：
本研究旨在对从大脑样本中获得的弓形虫分离株进行基因型分析，帕拉伊巴州供人类食用的山羊和绵羊的隔膜和心脏，巴西。14只动物的组织样本,山羊（n=5）和羔羊（n=9），来自七个城市的公共屠宰场，并在老鼠身上进行了生物检测。利用小鼠的脑进行DNA提取。通过聚合酶链反应限制性片段长度多态性（PCR-RFLP）使用10个标记（SAG1，SAG2，SAG3，BTUB，c22-8、PK1、GRA6、L358、c-29-2和Apico)。总共10个分离株被完全基因分型（即在所有基因座），三个来自山羊，七个来自绵羊，揭示了五种不同的基因型:#13(n=4);#48(n=3);#57(n=1);#273(n=1);和一种以前没有描述过的新基因型。基因型#13经常在巴西东北部发现,并且代表在该区域中循环的克隆谱系,并且是鉴定的最普遍的基因型(n=4)。此外，在本研究中，基因型#13、#48、#57和#273在巴西绵羊中首次被记录，新的基因型是从山羊中分离出来的。我们的发现与以前对巴西弓形虫的研究一致，不断发现新的基因型，突出了该国弓形虫分离株的高度遗传多样性。
