UNASSIGNED: A cell culture experimental study was carried out at Pharmacology department of Bahria University Medical and Dental College (January to June 2021) in collaboration with Aga Khan University, Karachi, Pakistan. Cell viability and proliferation assays were used to quantify dead and alive cells by utilizing a tetrazolium assay and an enzyme immunosorbent plate reader was used to calculate their absorbance. For the apoptosis initiation assay, these cells were dyed with a fluorescent stain and observed for fluorescence and apoptosis. During cell viability testing, various I. verum methanolic extract doses (0.125, 0.25, 0.5, 1, 3, 6, 12, and 25µg/ml) were employed to treat MDA-MB-231 cells, while the IC50 dose of 2.8µg/ml was used for both the cell proliferation and apoptosis initiation assays.
UNASSIGNED: In the cell viability assay, all I. verum methanolic extract doses exhibited a substantial decrease in the viability of MDA-MB-231 cells (less than 0.01 p-value). In cell proliferation assay and apoptosis initiation, the IC50 dose of 2.8µg/ml of I. verum methanolic extract also exhibited a substantial decrease in cell division (less than 0.01 p-value) and the initiation of apoptosis in MDA-MB-231 cells.
UNASSIGNED: Illicium verum methanolic extract have strong anticancer activity against triple-negative breast cancer MDA-MB-231 cell line through cytotoxicity, proliferation reduction, and apoptosis initiation mechanisms.
■与阿加汗大学合作,在Bahria大学医学院药理学系(2021年1月至6月)进行了细胞培养实验研究,卡拉奇,巴基斯坦。通过使用四唑盐测定法使用细胞活力和增殖测定法来定量死细胞和活细胞,并使用酶免疫吸附板读数器计算其吸光度。对于凋亡起始测定,这些细胞用荧光染色剂染色,观察荧光和凋亡。在细胞活力测试期间,使用各种I.verum甲醇提取物剂量(0.125、0.25、0.5、1、3、6、12和25µg/ml)来处理MDA-MB-231细胞,而2.8µg/ml的IC50剂量用于细胞增殖和凋亡起始测定。
■在细胞活力测定中,所有I.verum甲醇提取物剂量均显示MDA-MB-231细胞的活力显着降低(小于0.01p值)。在细胞增殖测定和凋亡启动中,在MDA-MB-231细胞中,2.8µg/mlI.verum甲醇提取物的IC50剂量也显示出细胞分裂显著减少(p值小于0.01)和细胞凋亡的启动。
■八角甲醇提取物通过细胞毒性对三阴性乳腺癌MDA-MB-231细胞系具有很强的抗癌活性,减少增殖,和凋亡启动机制。