PDF(Pubmed)
Abstract：
Macromolecular complexes play essential roles in various cellular processes. The assembly of macromolecular assemblies within the cell must overcome barriers imposed by a crowded cellular environment which is characterized by an estimated concentration of biological macromolecules amounting to 100-450 g/L that take up approximately 5-40% of the cytoplasmic volume. The formation of the macromolecular assemblies is facilitated by molecular chaperones in cooperation with their co-chaperones. The R2TP protein complex has emerged as a co-chaperone of Hsp90 that plays an important role in macromolecular assembly. The R2TP complex is composed of a heterodimer of RPAP3:P1H1DI that is in turn complexed to members of the ATPase associated with diverse cellular activities (AAA +), RUVBL1 and RUVBL2 (R1 and R2) families. What makes the R2TP co-chaperone complex particularly important is that it is involved in a wide variety of cellular processes including gene expression, translation, co-translational complex assembly, and posttranslational protein complex formation. The functional versatility of the R2TP co-chaperone complex makes it central to cellular development; hence, it is implicated in various human diseases. In addition, their roles in the development of infectious disease agents has become of interest. In the current review, we discuss the roles of these proteins as co-chaperones regulating Hsp90 and its partnership with Hsp70. Furthermore, we highlight the structure-function features of the individual proteins within the R2TP complex and describe their roles in various cellular processes.
摘要：
大分子复合物在各种细胞过程中起着重要作用。细胞内大分子组装体的组装必须克服由拥挤的细胞环境施加的障碍,其特征在于生物大分子的估计浓度为100-450g/L,其占据约5-40%的细胞质体积。大分子组装体的形成通过分子伴侣与它们的共伴侣协作来促进。R2TP蛋白复合物已成为Hsp90的共同伴侣，在大分子组装中起重要作用。R2TP复合物由RPAP3：P1H1DI的异二聚体组成，该二聚体又与与多种细胞活动（AAA）相关的ATPase成员复合。RUVBL1和RUVBL2(R1和R2)家族。使R2TP共同伴侣复合体特别重要的是，它涉及多种细胞过程，包括基因表达，翻译,协同平移复杂装配，和翻译后蛋白质复合物的形成。R2TP共同伴侣复合物的功能多功能性使其成为细胞发育的核心；因此，它与各种人类疾病有关。此外,它们在传染病病原体发展中的作用已经引起了人们的兴趣。在当前的审查中，我们讨论了这些蛋白质作为共同伴侣调节Hsp90的作用及其与Hsp70的伙伴关系。此外，我们强调了R2TP复合物中单个蛋白质的结构-功能特征，并描述了它们在各种细胞过程中的作用。
