Abstract：
BACKGROUND: Diabetes mellitus and depression are comorbidities that can be caused by each other. Brain-derived neurotrophic factor (BDNF) functions as a neuronal growth factor. It maintains the functional integrity of the nervous system.
OBJECTIVE: To study the possible association between BDNF levels and gene polymorphism with depression in patients diagnosed with type 2 diabetes mellitus.
METHODS: The Elisa technique measured BDNF, and rs6265 gene polymorphism was detected using real-time PCR. Depression was assessed utilizing a clinical interview tool designed to establish the diagnosis of depression and differentiate it from other psychiatric diseases.
RESULTS: BDNF levels were significantly lower in patients with type 2 diabetes mellitus and symptoms of depression than in patients with type 2 diabetes mellitus and no symptoms of depression (82.6±16.1. Vs 122± 17.47, p˂ 0.001). There was a statistically significant difference in BDNF levels in patients with diabetes among the three genotypes of the BDNF gene (p-value < 0.001). Val/ Val carriers had the highest serum BDNF levels, and Met/ Met carriers had the lowest serum BDNF levels. Subgroup analysis showed statistically significant genotype-related differences in serum BDNF levels among the three subgroups in the Depression group. Val/ Val carriers had the highest serum BDNF levels, and Met/ Met carriers had the lowest serum BDNF levels. BDNF Val66Met polymorphism had no significant association with the presence of depression, yet there was a trend towards significance (p = 0.05) Conclusion: In this pilot, Low levels of BDNF were associated with depression in patients with type 2 diabetes. Carriers of the Met/ Met allele have the lowest serum BDNF levels. Multicenter studies with more participants are required.
OBJECTIVE: To study the possible association between BDNF levels and gene polymorphism with depression in patients diagnosed with type 2 diabetes mellitus.
METHODS: The Elisa technique measured BDNF, and rs6265 gene polymorphism was detected using real-time PCR. Depression was assessed utilizing a clinical interview tool designed to establish the diagnosis of depression and differentiate it from other psychiatric diseases.
RESULTS: BDNF levels were significantly lower in patients with type 2 diabetes mellitus and symptoms of depression than in patients with type 2 diabetes mellitus and no symptoms of depression (82.6±16.1. Vs 122± 17.47, p˂ 0.001). There was a statistically significant difference in BDNF levels in patients with diabetes among the three genotypes of the BDNF gene (p-value < 0.001). Val/ Val carriers had the highest serum BDNF levels, and Met/ Met carriers had the lowest serum BDNF levels. Subgroup analysis showed statistically significant genotype-related differences in serum BDNF levels among the three subgroups in the Depression group. Val/ Val carriers had the highest serum BDNF levels, and Met/ Met carriers had the lowest serum BDNF levels. BDNF Val66Met polymorphism had no significant association with the presence of depression, yet there was a trend towards significance (p = 0.05) Conclusion: In this pilot, Low levels of BDNF were associated with depression in patients with type 2 diabetes. Carriers of the Met/ Met allele have the lowest serum BDNF levels. Multicenter studies with more participants are required.
摘要：
背景：糖尿病和抑郁症是可以相互引起的合并症。脑源性神经营养因子（BDNF）作为神经元生长因子发挥作用。它保持神经系统的功能完整性。
目的：研究2型糖尿病患者BDNF水平和基因多态性与抑郁症的可能关联。
方法：Elisa技术测量BDNF，rs6265基因多态性用实时荧光定量PCR检测。使用旨在确定抑郁症诊断并将其与其他精神疾病区分开的临床访谈工具评估抑郁症。
结果：有抑郁症状的2型糖尿病患者的BDNF水平明显低于无抑郁症状的2型糖尿病患者（82.6±16.1。Vs122±17.47，p=0.001）。在BDNF基因的三种基因型之间，糖尿病患者的BDNF水平存在统计学上的显着差异（p值<0.001）。Val/Val携带者的血清BDNF水平最高，Met/Met携带者的血清BDNF水平最低。亚组分析显示，抑郁症组三个亚组之间血清BDNF水平的基因型相关差异具有统计学意义。Val/Val携带者的血清BDNF水平最高，Met/Met携带者的血清BDNF水平最低。BDNFVal66Met多态性与抑郁症的存在无显著关联,然而有显着性的趋势(p=0.05)结论：在这个试点中，低水平的BDNF与2型糖尿病患者的抑郁有关。Met/Met等位基因的携带者具有最低的血清BDNF水平。需要有更多参与者的多中心研究。
目的：研究2型糖尿病患者BDNF水平和基因多态性与抑郁症的可能关联。
方法：Elisa技术测量BDNF，rs6265基因多态性用实时荧光定量PCR检测。使用旨在确定抑郁症诊断并将其与其他精神疾病区分开的临床访谈工具评估抑郁症。
结果：有抑郁症状的2型糖尿病患者的BDNF水平明显低于无抑郁症状的2型糖尿病患者（82.6±16.1。Vs122±17.47，p=0.001）。在BDNF基因的三种基因型之间，糖尿病患者的BDNF水平存在统计学上的显着差异（p值<0.001）。Val/Val携带者的血清BDNF水平最高，Met/Met携带者的血清BDNF水平最低。亚组分析显示，抑郁症组三个亚组之间血清BDNF水平的基因型相关差异具有统计学意义。Val/Val携带者的血清BDNF水平最高，Met/Met携带者的血清BDNF水平最低。BDNFVal66Met多态性与抑郁症的存在无显著关联,然而有显着性的趋势(p=0.05)结论：在这个试点中，低水平的BDNF与2型糖尿病患者的抑郁有关。Met/Met等位基因的携带者具有最低的血清BDNF水平。需要有更多参与者的多中心研究。
