Abstract:
Abiotic stress is one of the main threats affecting crop growth and production. Nitric oxide (NO), an important signaling molecule involved in wide range of plant growth and development as well as in response to abiotic stress. NO can exert its biological functions through protein S-nitrosylation, a redox-based posttranslational modification by covalently adding NO moiety to a reactive cysteine thiol of a target protein to form an S-nitrosothiol (SNO). Protein S-nitrosylation is an evolutionarily conserved mechanism regulating multiple aspects of cellular signaling in plant. Recently, emerging evidence have elucidated protein S-nitrosylation as a modulator of plant in responses to abiotic stress, including salt stress, extreme temperature stress, light stress, heavy metal and drought stress. In addition, significant mechanism has been made in functional characterization of protein S-nitrosylated candidates, such as changing protein conformation, and the subcellular localization of proteins, regulating protein activity and influencing protein interactions. In this study, we updated the data related to protein S-nitrosylation in plants in response to adversity and gained a deeper understanding of the functional changes of target proteins after protein S-nitrosylation.
摘要:
非生物胁迫是影响作物生长和生产的主要威胁之一。一氧化氮(NO),一种重要的信号分子,参与广泛的植物生长和发育以及对非生物胁迫的响应。NO可以通过蛋白质S-亚硝基化发挥其生物学功能,基于氧化还原的翻译后修饰,通过将NO部分共价添加至靶蛋白的反应性半胱氨酸硫醇以形成S-亚硝基硫醇(SNO)。蛋白质S-亚硝基化是调节植物细胞信号传导的多个方面的进化保守机制。最近,新的证据已经阐明了蛋白质S-亚硝基化作为植物响应非生物胁迫的调节剂,包括盐胁迫,极端温度应力,轻应力,重金属和干旱胁迫。此外,在蛋白质S-亚硝基化候选物的功能表征中已经取得了重要的机制,比如改变蛋白质构象,以及蛋白质的亚细胞定位,调节蛋白质活性和影响蛋白质相互作用。在这项研究中,我们更新了植物在逆境中与蛋白质S-亚硝基化相关的数据,并对蛋白质S-亚硝基化后靶蛋白的功能变化有了更深入的了解。