PDF(Pubmed)
Abstract：
BACKGROUND: Vector sand fly colonies are a critical component of studies aimed at improving the understanding of the neglected tropical disease leishmaniasis and alleviating its global impact. However, among laboratory-colonized arthropod vectors of infectious diseases, the labor-intensive nature of sand fly rearing coupled with the low number of colonies worldwide has generally discouraged the widespread use of sand flies in laboratory settings. Among the different factors associated with the low productivity of sand fly colonies, mite infestations are a significant factor. Sand fly colonies are prone to infestation by mites, and the physical interactions between sand flies and mites and metabolites have a negative impact on sand fly larval development.
METHODS: Mites were collected from sand fly larval rearing pots and morphologically identified using taxonomic keys. Upon identification, they were photographed with a scanning electron microscope. Several mite control measures were adopted in two different laboratories, one at the Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases-National Institutes of Health (Rockville, MD, USA), and the other at the University of Calgary (Calgary, AB, Canada).
RESULTS: The mite species associated with sand fly colonies in the two laboratories were morphologically identified as Tyrophagus sp. and Stratiolaelaps scimitus. While complete eradication of mites in sand fly colonies is considered unrealistic, drastically reducing their population has been associated with higher sand fly productivity.
CONCLUSIONS: We report a case of detrimental interaction between sand flies and Tyrophagus sp. and S. scimitus in a closed laboratory sand fly colony, discuss their impact on sand fly production and provide guidelines for limiting the mite population size in a closed laboratory colony leading to improved sand fly yields.
METHODS: Mites were collected from sand fly larval rearing pots and morphologically identified using taxonomic keys. Upon identification, they were photographed with a scanning electron microscope. Several mite control measures were adopted in two different laboratories, one at the Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases-National Institutes of Health (Rockville, MD, USA), and the other at the University of Calgary (Calgary, AB, Canada).
RESULTS: The mite species associated with sand fly colonies in the two laboratories were morphologically identified as Tyrophagus sp. and Stratiolaelaps scimitus. While complete eradication of mites in sand fly colonies is considered unrealistic, drastically reducing their population has been associated with higher sand fly productivity.
CONCLUSIONS: We report a case of detrimental interaction between sand flies and Tyrophagus sp. and S. scimitus in a closed laboratory sand fly colony, discuss their impact on sand fly production and provide guidelines for limiting the mite population size in a closed laboratory colony leading to improved sand fly yields.
摘要：
背景：媒介沙蝇菌落是旨在提高对被忽视的热带病利什曼病的理解并减轻其全球影响的研究的关键组成部分。然而,在传染病的实验室定植的节肢动物媒介中，沙蝇饲养的劳动密集型性质加上全球殖民地数量少，通常阻碍了沙蝇在实验室环境中的广泛使用。在与沙蝇菌落生产力低相关的不同因素中，螨感染是一个重要因素。沙蝇群容易受到螨虫侵扰,沙蝇与螨虫和代谢物之间的物理相互作用对沙蝇幼虫的发育有负面影响。
方法：从沙蝇幼虫饲养盆中收集螨，并使用分类学键进行形态鉴定。识别后，他们用扫描电子显微镜拍照。两个不同的实验室采取了几种防治螨的措施,一个在疟疾和病媒研究实验室，国家过敏和传染病研究所-国立卫生研究院（罗克维尔，MD,美国),另一个在卡尔加里大学(卡尔加里，AB,加拿大)。
结果：在两个实验室中与沙蝇菌落相关的螨物种在形态上被鉴定为Tyrophagussp。和Stratiolaelapsscimitus。虽然完全根除沙蝇群落中的螨虫被认为是不现实的，人口的急剧减少与更高的沙蝇生产力有关。
结论：我们报告了一例沙蝇和Tyrophagus之间的有害相互作用。和S.scimitus在一个封闭的实验室沙蝇殖民地，讨论它们对沙蝇生产的影响，并提供在封闭的实验室菌落中限制螨种群大小的指导方针，从而提高沙蝇的产量。
方法：从沙蝇幼虫饲养盆中收集螨，并使用分类学键进行形态鉴定。识别后，他们用扫描电子显微镜拍照。两个不同的实验室采取了几种防治螨的措施,一个在疟疾和病媒研究实验室，国家过敏和传染病研究所-国立卫生研究院（罗克维尔，MD,美国),另一个在卡尔加里大学(卡尔加里，AB,加拿大)。
结果：在两个实验室中与沙蝇菌落相关的螨物种在形态上被鉴定为Tyrophagussp。和Stratiolaelapsscimitus。虽然完全根除沙蝇群落中的螨虫被认为是不现实的，人口的急剧减少与更高的沙蝇生产力有关。
结论：我们报告了一例沙蝇和Tyrophagus之间的有害相互作用。和S.scimitus在一个封闭的实验室沙蝇殖民地，讨论它们对沙蝇生产的影响，并提供在封闭的实验室菌落中限制螨种群大小的指导方针，从而提高沙蝇的产量。
