Abstract：
Two new compounds eutyditerpenoid A (1) and seco-phenochalasin B (5), together with seven known compounds diaporthein A (2), aspergillon A (3), phenochalasin B (4), cytochalasins Z24 and Z25 (6 and 7), scoparasins A and B (8 and 9) were isolated from marine-derived Eutypella scoparia GZU-4-19Y. Among them, eutyditerpenoid A (1) with a rare 6/7/6 ring system possesing an anhydride moiety was the first example in the pimarane-type diterpenoids. Their structures were determined based on spectroscopic methods and the electronic circular dichroism (ECD) calculations. In the bioassays, all of the isolates were evaluated for their inhibitory activity against NO production induced by lipopolysaccharide in RAW 264.7 cells. Compounds 3 and 7 showed potent NO inhibition activity with IC50 values of 2.1 and 17.1 μM respectively, and the former also significantly suppressed the protein expression of iNOS and COX-2 at the concentration of 2.5 μM.
摘要：
两个新的化合物Eutyd2萜A（1）和Seco-phochalasinB（5），连同7种已知的化合物，aspergillonA(3),phochalasinB(4),细胞松弛素Z24和Z25（6和7），scoparasinA和B（8和9）从海洋来源的EutypellascopariaGZU-4-19Y中分离出来。其中,具有罕见的6/7/6环系统的具有酸酐部分的戊二萜A（1）是匹马环型二萜中的第一个例子。它们的结构是根据光谱法和电子圆二色性（ECD）计算确定的。在生物测定中，在RAW264.7细胞中评估了所有分离株对脂多糖诱导的NO产生的抑制活性。化合物3和7显示出有效的NO抑制活性，IC50值分别为2.1和17.1μM，并且前者在2.5μM的浓度下还显着抑制iNOS和COX-2的蛋白表达。
