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Abstract：
In order to investigate and study the species and distribution of freshwater snails in Ordos area of Inner Mongolia, as well as the trematode infection in different periods, and to provide a scientific basis for the effective prevention and control of livestock trematodiasis. In this paper, freshwater snails distributed in Ordos were widely collected for morphological identification, and PCR amplification of freshwater snails COI gene and ITS2 gene was carried out with the help of molecular biology. At the same time, microscopic examination was used to observe the trematode infection of freshwater snails in two different periods from May to July and July to September, and the molecular biology of the trematodes was identified. The results showed that the 1796 freshwater snails collected belonged to two orders, three families and four genera, i.e. Bellamya, Radix, Galba, and Gyraulus. Microscopic examination of snails showed that the infection rate of trematode larvae from July to September was significantly higher than that from May to July. The collected trematodes were identified as five species, namely Cotylurus marcogliesei, Fasciola hepatica, Fasciola gigantica, Paramphistomum cervi, and Parastrigea robusta. The combination of freshwater snail species in Ordos and the infection of trematode in snails showed that a large number of freshwater snails were infected with trematodes, especially from July to September, when there is more rain and suitable climate, which causes serious harm to local livestock.
摘要：
为了调查和研究内蒙古鄂尔多斯地区淡水蜗牛的种类和分布,以及不同时期的吸虫感染，为有效防治家畜吸虫病提供科学依据。在本文中,广泛采集分布在鄂尔多斯的淡水蜗牛进行形态鉴定，利用分子生物学技术对淡水蜗牛COI基因和ITS2基因进行PCR扩增。同时,在5月至7月和7月至9月的两个不同时期，采用显微镜检查观察淡水蜗牛的吸虫感染情况，并鉴定了吸虫的分子生物学。结果表明，收集的1796个淡水蜗牛属于两个订单，三个家庭和四个属，即贝拉米亚，Radix,Galba,和轮回。蜗牛的显微镜检查显示，7-9月吸虫幼虫的感染率明显高于5-7月。收集的吸虫被鉴定为五种，也就是马可利西毛,肝片吸虫,巨大的Fasciola,两栖动物宫颈，和Parastrigearobusta.鄂尔多斯淡水蜗牛种类和蜗牛吸虫感染的组合表明,大量淡水蜗牛感染吸虫,尤其是7月到9月,当有更多的降雨和合适的气候时，对当地牲畜造成严重危害。
