Abstract：
OBJECTIVE: The removal of impacted third molars by surgery may occur with a series of complications, whereas limited information about the postoperative pathogenesis is available. The objective of this study is to identify changes in gene expression after flap surgical removal of impacted third molars and provide potential information to reduce postoperative complications.
METHODS: The gingival tissues of twenty patients with flap surgical removal of impacted third molars and twenty healthy volunteers were collected for gene expression testing. The collected gingival tissues were used RNA sequencing technology and quantitative real-time PCR validation was performed. DEG was mapped to protein databases such as GO and KEGG for functional annotation and, based on annotation information, for mining of differential expression genes in patients with mpacted third molars.
RESULTS: A total of 555 genes were differentially expressed. Among the top up-regulated genes, HLA-DRB4, CCL20, and CXCL8 were strongly associated with immune response and signal transduction. Among the top down-regulated genes, SPRR2B, CLDN17, LCE3D and LCE3E were related to keratinocyte differentiation, IFITM5, and BGLAP were related to bone mineralization, UGT2B17 is associated with susceptibility to osteoporosis. KEGG results showed that the DEGs were related to multiple disease-related pathways.
CONCLUSIONS: This first transcriptome analysis of gingival tissues from patients with surgical removal of impacted third molars provides new insights into postoperative genetic changes. The results may establish a basis for future research on minimizing the incidence of complications after flap-treated third molars.
METHODS: The gingival tissues of twenty patients with flap surgical removal of impacted third molars and twenty healthy volunteers were collected for gene expression testing. The collected gingival tissues were used RNA sequencing technology and quantitative real-time PCR validation was performed. DEG was mapped to protein databases such as GO and KEGG for functional annotation and, based on annotation information, for mining of differential expression genes in patients with mpacted third molars.
RESULTS: A total of 555 genes were differentially expressed. Among the top up-regulated genes, HLA-DRB4, CCL20, and CXCL8 were strongly associated with immune response and signal transduction. Among the top down-regulated genes, SPRR2B, CLDN17, LCE3D and LCE3E were related to keratinocyte differentiation, IFITM5, and BGLAP were related to bone mineralization, UGT2B17 is associated with susceptibility to osteoporosis. KEGG results showed that the DEGs were related to multiple disease-related pathways.
CONCLUSIONS: This first transcriptome analysis of gingival tissues from patients with surgical removal of impacted third molars provides new insights into postoperative genetic changes. The results may establish a basis for future research on minimizing the incidence of complications after flap-treated third molars.
摘要：
目的：手术切除阻生第三磨牙可能会发生一系列并发症，而关于术后发病机制的信息有限。本研究的目的是确定皮瓣手术切除阻生第三磨牙后基因表达的变化，并为减少术后并发症提供潜在信息。
方法：收集20例皮瓣切除阻生第三磨牙患者和20例健康志愿者的牙龈组织进行基因表达检测。采用RNA测序技术采集牙龈组织,并进行实时定量PCR验证。DEG被映射到蛋白质数据库，如GO和KEGG进行功能注释，基于注释信息，挖掘第三磨牙畸形患者的差异表达基因。
结果：共555个基因差异表达。在最高调控基因中，HLA-DRB4、CCL20和CXCL8与免疫应答和信号转导密切相关。在顶部下调的基因中，SPRR2B,CLDN17，LCE3D和LCE3E与角质形成细胞分化有关，FITM5和BGLAP与骨矿化有关，UGT2B17与骨质疏松症易感性相关。KEGG结果表明，DEGs与多种疾病相关途径有关。
结论：对手术切除阻生第三磨牙患者牙龈组织的首次转录组分析提供了对术后遗传变化的新见解。该结果可能为将来减少皮瓣治疗第三磨牙后并发症发生率的研究奠定基础。
方法：收集20例皮瓣切除阻生第三磨牙患者和20例健康志愿者的牙龈组织进行基因表达检测。采用RNA测序技术采集牙龈组织,并进行实时定量PCR验证。DEG被映射到蛋白质数据库，如GO和KEGG进行功能注释，基于注释信息，挖掘第三磨牙畸形患者的差异表达基因。
结果：共555个基因差异表达。在最高调控基因中，HLA-DRB4、CCL20和CXCL8与免疫应答和信号转导密切相关。在顶部下调的基因中，SPRR2B,CLDN17，LCE3D和LCE3E与角质形成细胞分化有关，FITM5和BGLAP与骨矿化有关，UGT2B17与骨质疏松症易感性相关。KEGG结果表明，DEGs与多种疾病相关途径有关。
结论：对手术切除阻生第三磨牙患者牙龈组织的首次转录组分析提供了对术后遗传变化的新见解。该结果可能为将来减少皮瓣治疗第三磨牙后并发症发生率的研究奠定基础。
