PDF(Pubmed)
Abstract：
The human gut microbiome plays a significant role in health and disease. The viral component (virome) is predominantly composed of bacteriophages (phages) and has received significantly less attention in comparison to the bacteriome. This knowledge gap is largely due to challenges associated with the isolation and characterization of novel gut phages, and bioinformatic hurdles such as the lack of a universal phage marker gene and the absence of sufficient numbers of homologs in viral databases. Here, we describe the isolation from human feces of a novel lytic phage with siphovirus morphology, φPDS1, infecting Parabacteroides distasonis APCS2/PD, and classified within a newly proposed Sagittacolavirus genus. In silico and biological characterization of this phage is presented in this study. Key to the isolation of φPDS1 was the antibiotic-driven selective enrichment of the bacterial host in a fecal fermenter. Despite producing plaques and lacking genes associated with lysogeny, φPDS1 demonstrates the ability to coexist in liquid culture for multiple days without affecting the abundance of its host. Multiple studies have shown that changes in Parabacteroides distasonis abundance can be linked to various disease states, rendering this novel phage-host pair and their interactions of particular interest.
摘要：
人类肠道微生物组在健康和疾病中起着重要作用。病毒组分（病毒体）主要由噬菌体（噬菌体）组成，与细菌组相比，受到的关注明显较少。这种知识差距很大程度上是由于与新型肠道噬菌体的隔离和表征相关的挑战。和生物信息学障碍，例如缺乏通用的噬菌体标记基因和病毒数据库中缺乏足够数量的同源物。这里,我们描述了从人类粪便中分离出一种具有syphovirus形态的新型裂解噬菌体，φPDS1，感染分离副杆菌APCS2/PD，并归入新提出的矢状病毒属。本研究介绍了该噬菌体的计算机模拟和生物学特性。分离φPDS1的关键是粪便发酵罐中细菌宿主的抗生素驱动选择性富集。尽管产生斑块并且缺乏与溶源性相关的基因，φPDS1证明了在液体培养中共存多天而不影响其宿主丰度的能力。多项研究表明，分离副杆菌丰度的变化可能与各种疾病状态有关，呈现这种新的噬菌体-宿主对及其特别感兴趣的相互作用。
