Abstract：
The release of isoprene by plants is considered to be an adaptation to the environment. Herein, a highly selective coumarin fluorescent probe (DMIC) was designed for detecting isoprene. When isoprene came into contact with the maleimide of DMIC, an electrophilic addition process took place. The powerful push-pull effect of DMIC was disrupted. Simultaneously, intramolecular charge transfer was initiated. This enabled DMIC to achieve rapid detection of isoprene within 5 min. Furthermore, excellent linearity was observed in the concentration range of 1-560 ppm (R2 = 0.996). A limit of detection is 1.6 ppm. DMIC was applied to in vitro studies of plant release of liberated isoprene. By monitoring the release of isoprene from different tree species throughout the day, the dynamics of isoprene release from plants throughout the day have been successfully revealed. In addition, the release of isoprene varied considerably among different tree species. In particular, the biocompatibility of DMIC allowed for the in vivo detection of isoprene using fluorescence imaging. The results successfully revealed the dynamics of isoprene release in plants under stress. The amount of isoprene that a plant produced increased with the severity of the stress it experienced. This suggested that the level of isoprene content in plants could be used as a preliminary indicator of the physiological health status of plants. This research demonstrates great potential for clarifying signal transduction in biological systems. It provided ideas for further understanding the biology of isoprene.
摘要：
植物释放异戊二烯被认为是对环境的适应。在这里，设计了一种用于异戊二烯检测的高选择性香豆素荧光探针(DMIC)。当异戊二烯与DMIC的马来酰亚胺接触时，发生了亲电子加成过程。DMIC强大的推拉效应被破坏。同时,分子内电荷转移开始。这使得DMIC能够在5分钟内实现异戊二烯的快速检测。此外，在1-560ppm（R2=0.996）的浓度范围内观察到出色的线性。检测极限为1.6ppm。将DMIC应用于释放的异戊二烯的植物释放的体外研究。通过全天监测不同树种的异戊二烯释放，已经成功揭示了植物全天释放异戊二烯的动力学。此外,异戊二烯的释放在不同树种之间差异很大。特别是,DMIC的生物相容性允许使用荧光成像在体内检测异戊二烯。结果成功地揭示了胁迫下植物中异戊二烯释放的动力学。植物产生的异戊二烯的量随着其经历的压力的严重程度而增加。这表明植物中异戊二烯含量的水平可以作为植物生理健康状况的初步指标。这项研究证明了阐明生物系统中信号转导的巨大潜力。为进一步了解异戊二烯的生物学行为提供了思路。
