Abstract：
Despite 40 years of development of DNA nanotechnology, the fundamental knowledge of the process of DNA strand assembly into targeted nanostructures remains unclear. Study of the dynamic process, especially the competing hybridizations in kinetic traps, provides insight into DNA assembly. In this study, a system of middle-domain first assembly (MDFA) was proposed to enable oligonucleotides to assemble into a 2D DNA monolayer in a pathway-dependent approach. This system was an ideal case to study the dynamic interactions between competing hybridizations during oligonucleotide assembly. Dynamic study revealed the coexistence of the kinetically trapped dead-end byproduct and target product at the early stage of annealing, followed by transformation of the byproduct into the target product by reverse disassembly, due to the equilibrium of the competing hybridizations increasingly favoring the target product pathway. This study offered a better understanding of the assembly pathway of DNA nanostructures for future design.
摘要：
尽管DNA纳米技术发展了40年,DNA链组装成靶向纳米结构的过程的基本知识仍不清楚.研究动态过程，尤其是动力学陷阱中的竞争杂交，提供了对DNA组装的见解。在这项研究中,提出了一种中间结构域首次组装（MDFA）系统，以使寡核苷酸能够以途径依赖性方法组装成2DDNA单层。该系统是研究寡核苷酸组装过程中竞争性杂交之间的动态相互作用的理想情况。动态研究表明，退火初期动力学捕获的死端副产物和目标产物共存，然后通过反向拆卸将副产品转化为目标产品，由于竞争杂交的平衡越来越有利于目标产物途径。这项研究为将来的设计提供了对DNA纳米结构组装途径的更好理解。
