Abstract：
UNASSIGNED: Knot configuration is an important but relatively neglected topic in microvascular anastomosis literature.
UNASSIGNED: To study the differences between end-to-end microvascular anastomosis performed with two-throw reef knots as compared to traditional three-throw knots in a rat femoral artery model at the histological level.
UNASSIGNED: Sprague Dawley rats underwent end-to-end microvascular anastomosis of the right femoral artery (one-way-up method). The rats were divided into two groups: two-throw reef knots versus traditional three-throw knots. The patency was checked by the standard empty refill method. After 2 weeks, the rats underwent re-exploration. An anastomotic segment was sent for histological analysis. Histological alterations including luminal patency and changes in Tunica intima, Tunica media, and Tunica adventitia were compared between the two groups.
UNASSIGNED: Twenty-nine rats were operated on by the senior author (17 by three-throw and 12 by two-throw reef knots). In the two-throw reef knot group versus the traditional three-throw knot group, the immediate patency rates were 100% versus 82.4%, and the delayed patency rates were 90.9% versus 62.5%, respectively. The histopathological patency rates were concordant with delayed patency rates. Subintimal proliferation and fibrosis were comparable in both groups. Adventitial granulomas were noted in all, irrespective of the knotting technique. Tunica media preservation rates for the two-throw reef knot versus the traditional three-throw knot group were 63.6% versus 0%. Five rats were operated by the beginner in the field, all by two-throw reef knots (to assess the safety of this new method in the hands of a beginner).
UNASSIGNED: Microvascular anastomosis performed with two-throw reef knots appears not only feasible but better in terms of anastomosis patency. Histological superiority in terms of Tunica media preservation further validates the technique.
UNASSIGNED: To study the differences between end-to-end microvascular anastomosis performed with two-throw reef knots as compared to traditional three-throw knots in a rat femoral artery model at the histological level.
UNASSIGNED: Sprague Dawley rats underwent end-to-end microvascular anastomosis of the right femoral artery (one-way-up method). The rats were divided into two groups: two-throw reef knots versus traditional three-throw knots. The patency was checked by the standard empty refill method. After 2 weeks, the rats underwent re-exploration. An anastomotic segment was sent for histological analysis. Histological alterations including luminal patency and changes in Tunica intima, Tunica media, and Tunica adventitia were compared between the two groups.
UNASSIGNED: Twenty-nine rats were operated on by the senior author (17 by three-throw and 12 by two-throw reef knots). In the two-throw reef knot group versus the traditional three-throw knot group, the immediate patency rates were 100% versus 82.4%, and the delayed patency rates were 90.9% versus 62.5%, respectively. The histopathological patency rates were concordant with delayed patency rates. Subintimal proliferation and fibrosis were comparable in both groups. Adventitial granulomas were noted in all, irrespective of the knotting technique. Tunica media preservation rates for the two-throw reef knot versus the traditional three-throw knot group were 63.6% versus 0%. Five rats were operated by the beginner in the field, all by two-throw reef knots (to assess the safety of this new method in the hands of a beginner).
UNASSIGNED: Microvascular anastomosis performed with two-throw reef knots appears not only feasible but better in terms of anastomosis patency. Histological superiority in terms of Tunica media preservation further validates the technique.
摘要：
■结构型是微血管吻合文献中一个重要但相对被忽视的话题。
■为了研究在组织学水平的大鼠股动脉模型中，与传统的三掷结相比，使用两掷礁结所进行的端到端微血管吻合之间的差异。
■SpragueDawley大鼠进行了右股动脉的端到端微血管吻合（单向向上方法）。将大鼠分为两组：两掷礁结和传统的三掷结。通过标准的空再填充方法检查通畅性。2周后,老鼠经历了重新探索。吻合段被送去进行组织学分析。组织学改变，包括管腔通畅和内膜改变，图尼卡媒体，比较两组的血管外膜和外膜。
■由资深作者对29只大鼠进行了手术（17只三掷，12只两掷礁结）。在两掷礁结组与传统的三掷结组中，即时通畅率为100%，而非82.4%，延迟通畅率分别为90.9%和62.5%，分别。组织病理学通畅率与延迟通畅率一致。两组的子内膜增生和纤维化具有可比性。全部可见外膜肉芽肿，不管打结技术如何。两掷礁结与传统三掷结组的膜介质保存率分别为63.6％和0％。初学者在野外操作了五只老鼠，全部通过两次投掷的礁石结（以评估初学者手中这种新方法的安全性）。
■用双掷触礁结进行的微血管吻合不仅可行，而且在吻合通畅性方面更好。在Tunica培养基保存方面的组织学优势进一步验证了该技术。
■为了研究在组织学水平的大鼠股动脉模型中，与传统的三掷结相比，使用两掷礁结所进行的端到端微血管吻合之间的差异。
■SpragueDawley大鼠进行了右股动脉的端到端微血管吻合（单向向上方法）。将大鼠分为两组：两掷礁结和传统的三掷结。通过标准的空再填充方法检查通畅性。2周后,老鼠经历了重新探索。吻合段被送去进行组织学分析。组织学改变，包括管腔通畅和内膜改变，图尼卡媒体，比较两组的血管外膜和外膜。
■由资深作者对29只大鼠进行了手术（17只三掷，12只两掷礁结）。在两掷礁结组与传统的三掷结组中，即时通畅率为100%，而非82.4%，延迟通畅率分别为90.9%和62.5%，分别。组织病理学通畅率与延迟通畅率一致。两组的子内膜增生和纤维化具有可比性。全部可见外膜肉芽肿，不管打结技术如何。两掷礁结与传统三掷结组的膜介质保存率分别为63.6％和0％。初学者在野外操作了五只老鼠，全部通过两次投掷的礁石结（以评估初学者手中这种新方法的安全性）。
■用双掷触礁结进行的微血管吻合不仅可行，而且在吻合通畅性方面更好。在Tunica培养基保存方面的组织学优势进一步验证了该技术。
