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Abstract：
World Organization for Animal Health has listed bluetongue (BT) under notifiable diseases. The BT is an arboviral infectious disease of domestic and wild ruminants caused by the bluetongue virus (BTV). Southern states of India had remained the point of attention for BT since first presence in 1964 in Maharashtra. Recently, northern states of India have also been reported positive for BTV in small ruminants. The present study reported the dual infection of BTV serotypes, BTV-12 and -16 in sheep population from Sirsa district of Haryana in the year 2016. After detection and serotyping with Seg-2 specific real time polymerase chain reaction (PCR), the Seg-2 and Seg-6 of BTV were PCR amplified and sequenced. On phylogenetic analysis it was detected to be clustered in nucleotype G and nucleotype B specific for BTV-12 and BTV-16, respectively. This was the first report of BTV-16 from Haryana. The results signified the co-infection of two different serotypes in an animal from a single outbreak.
摘要：
世界动物卫生组织已将蓝舌病（BT）列为应报告疾病。BT是由蓝舌病毒（BTV）引起的家养和野生反刍动物的虫媒病毒传染病。自1964年首次在马哈拉施特拉邦成立以来，印度南部各州一直是BT的关注点。最近,据报道，印度北部各州的小反刍动物BTV呈阳性。本研究报道了BTV血清型的双重感染，2016年哈里亚纳邦Sirsa区绵羊种群中的BTV-12和-16。在用Seg-2特异性实时聚合酶链反应（PCR）检测和血清分型后，对BTV的Seg-2和Seg-6进行PCR扩增和测序。在系统发育分析中，检测到它们分别聚集在对BTV-12和BTV-16具有特异性的核型G和核型B中。这是哈里亚纳邦BTV-16的第一份报告。结果表明来自单次爆发的动物中两种不同血清型的共感染。
