PDF(Pubmed)
Abstract：
BACKGROUND: Filamentous fungi are prolific producers of bioactive molecules and enzymes with important applications in industry. Yet, the vast majority of fungal species remain undiscovered or uncharacterized. Here we focus our attention to a wild fungal isolate that we identified as Anthostomella pinea. The fungus belongs to a complex polyphyletic genus in the family of Xylariaceae, which is known to comprise endophytic and pathogenic fungi that produce a plethora of interesting secondary metabolites. Despite that, Anthostomella is largely understudied and only two species have been fully sequenced and characterized at a genomic level.
RESULTS: In this work, we used long-read sequencing to obtain the complete 53.7 Mb genome sequence including the full mitochondrial DNA. We performed extensive structural and functional annotation of coding sequences, including genes encoding enzymes with potential applications in biotechnology. Among others, we found that the genome of A. pinea encodes 91 biosynthetic gene clusters, more than 600 CAZymes, and 164 P450s. Furthermore, untargeted metabolomics and molecular networking analysis of the cultivation extracts revealed a rich secondary metabolism, and in particular an abundance of sesquiterpenoids and sesquiterpene lactones. We also identified the polyketide antibiotic xanthoepocin, to which we attribute the anti-Gram-positive effect of the extracts that we observed in antibacterial plate assays.
CONCLUSIONS: Taken together, our results provide a first glimpse into the potential of Anthstomella pinea to provide new bioactive molecules and biocatalysts and will facilitate future research into these valuable metabolites.
RESULTS: In this work, we used long-read sequencing to obtain the complete 53.7 Mb genome sequence including the full mitochondrial DNA. We performed extensive structural and functional annotation of coding sequences, including genes encoding enzymes with potential applications in biotechnology. Among others, we found that the genome of A. pinea encodes 91 biosynthetic gene clusters, more than 600 CAZymes, and 164 P450s. Furthermore, untargeted metabolomics and molecular networking analysis of the cultivation extracts revealed a rich secondary metabolism, and in particular an abundance of sesquiterpenoids and sesquiterpene lactones. We also identified the polyketide antibiotic xanthoepocin, to which we attribute the anti-Gram-positive effect of the extracts that we observed in antibacterial plate assays.
CONCLUSIONS: Taken together, our results provide a first glimpse into the potential of Anthstomella pinea to provide new bioactive molecules and biocatalysts and will facilitate future research into these valuable metabolites.
摘要：
背景：丝状真菌是生物活性分子和酶的多产生产者，在工业中具有重要应用。然而，绝大多数真菌物种仍未被发现或未表征。在这里，我们将注意力集中在野生真菌分离株上，我们将其鉴定为Anthostomellapinea。该真菌属于木参科中的复杂多系属，已知其包含产生大量感兴趣的次生代谢产物的内生和致病真菌。尽管如此,对Anthostomella的研究不足，只有两个物种在基因组水平上进行了完全测序和表征。
结果：在这项工作中，我们使用长读数测序获得完整的53.7Mb基因组序列,包括完整的线粒体DNA.我们对编码序列进行了广泛的结构和功能注释，包括编码在生物技术中具有潜在应用的酶的基因。其中,我们发现A.pinea的基因组编码91个生物合成基因簇，超过600CAZymes,和164个P450。此外，培养提取物的非靶向代谢组学和分子网络分析揭示了丰富的次生代谢，特别是丰富的倍半萜类和倍半萜内酯。我们还确定了聚酮抗生素黄霉素，我们将在抗菌平板试验中观察到的提取物的抗革兰氏阳性作用归因于此。
结论：综合来看，我们的研究结果首次揭示了Anthstomellapinea提供新的生物活性分子和生物催化剂的潜力，并将促进对这些有价值的代谢物的未来研究。
结果：在这项工作中，我们使用长读数测序获得完整的53.7Mb基因组序列,包括完整的线粒体DNA.我们对编码序列进行了广泛的结构和功能注释，包括编码在生物技术中具有潜在应用的酶的基因。其中,我们发现A.pinea的基因组编码91个生物合成基因簇，超过600CAZymes,和164个P450。此外，培养提取物的非靶向代谢组学和分子网络分析揭示了丰富的次生代谢，特别是丰富的倍半萜类和倍半萜内酯。我们还确定了聚酮抗生素黄霉素，我们将在抗菌平板试验中观察到的提取物的抗革兰氏阳性作用归因于此。
结论：综合来看，我们的研究结果首次揭示了Anthstomellapinea提供新的生物活性分子和生物催化剂的潜力，并将促进对这些有价值的代谢物的未来研究。
