Abstract：
The genus Nocardia comprises gram-positive bacteria, most of which are pathogenic and cause opportunistic infections of the lungs, skin, and brain in humans. Based on a collaboration study with the Medical Mycology Research Center, Chiba University, we focused on Nocardia actinomycetes as a new natural-product resource. First, by culturing (monoculture) Nocardia in various media, we isolated a new aminocyclitol nabscessin A from Nocardia abscessus IFM10029T and a new γ-lactone inohanalactone from Nocardia inohanaensis IFM0092T. On the other hand, by imitating the state in which the genus Nocardia actinomycete infects animal cells and culturing the genus in the presence of animal cells (coculture), this genus was expected to produce new compounds through interactions with the animal cells. Using mouse macrophage-like cells (J774.1) as animal cells, a new pantothenic acid amide derivative and a cyclic peptide, nocarjamide, with Wnt signal activation activity were isolated from Nocardia tenerifensis IFM10554T strain.
摘要：
诺卡氏菌属包括革兰氏阳性菌，其中大多数是致病性的，会导致肺部的机会性感染，皮肤,人类的大脑。根据与医学肿瘤学研究中心的合作研究，千叶大学,我们专注于诺卡氏菌放线菌作为一种新的天然产物资源。首先,通过在各种培养基中培养（单一培养）诺卡氏菌，我们从诺卡氏菌脓肿IFM10029T中分离出一种新的氨基环醇囊素A，并从诺卡氏菌IFM0092T中分离出一种新的γ-内酯。另一方面,通过模仿诺卡氏菌放线菌属感染动物细胞的状态，并在动物细胞存在下培养该属（共培养），该属有望通过与动物细胞的相互作用产生新的化合物。使用小鼠巨噬细胞样细胞（J774.1）作为动物细胞，一种新的泛酸酰胺衍生物和一种环肽，诺卡贾德,具有Wnt信号激活活性的诺卡氏菌IFM10554T菌株。
