Abstract:
Cases of blastomycosis, a serious fungal disease globally rare but endemic to North America, can appear both sporadically and in outbreaks. Tracing these outbreaks to their environment has traditionally used culturing and polymerase chain reaction. Here, we present our method for metagenomic detection of Blastomyces in a 2015 outbreak soil sample from central Wisconsin. By sequencing this sample to multiple depths, we simulated the minimum required depth to detect Blastomyces in this outbreak. Our methods and recommendations can be used to identify the sources of blastomycosis during outbreaks and to learn about the ecology of Blastomyces.
摘要:
芽生菌病病例,一种全球罕见但北美特有的严重真菌病,可以偶尔出现和爆发。传统上使用培养和聚合酶链反应(PCR)将这些爆发追踪到他们的环境。这里,我们介绍了在威斯康星州中部2015年爆发的土壤样本中检测胚芽的宏基因组方法。通过将样本测序到多个深度,我们模拟了在这次爆发中检测胚芽所需的最小深度。我们的方法和建议可用于在暴发期间确定芽生菌病的来源,并了解芽生菌的生态学。
芽生菌病是一种严重的,但很罕见,在环境中难以发现的真菌感染。我们描述了一种通过与芽生菌病爆发相关的土壤的宏基因组测序鉴定芽生菌的新方法。这可能允许进一步研究其生态位。