PDF(Pubmed)
Abstract：
Reactive oxygen species (ROS) act as a group of signaling molecules in rice functioning in regulation of development and stress responses. Respiratory burst oxidase homologues (Rbohs) are key enzymes in generation of ROS. However, the role of the nine Rboh family members was not fully understood in rice multiple disease resistance and yield traits. In this study, we constructed mutants of each Rboh genes and detected their requirement in rice multiple disease resistance and yield traits. Our results revealed that mutations of five Rboh genes (RbohA, RbohB, RbohE, RbohH, and RbohI) lead to compromised rice blast disease resistance in a disease nursery and lab conditions; mutations of five Rbohs (RbohA, RbohB, RbohC, RbohE, and RbohH) result in suppressed rice sheath blight resistance in a disease nursery and lab conditions; mutations of six Rbohs (RbohA, RbohB, RbohC, RbohE, RbohH and RbohI) lead to decreased rice leaf blight resistance in a paddy yard and ROS production induced by PAMPs and pathogen. Moreover, all Rboh genes participate in the regulation of rice yield traits, for all rboh mutants display one or more compromised yield traits, such as panicle number, grain number per panicle, seed setting rate, and grain weight, resulting in reduced yield per plant except rbohb and rbohf. Our results identified the Rboh family members involved in the regulation of rice resistance against multiple pathogens that caused the most serious diseases worldwide and provide theoretical supporting for breeding application of these Rbohs to coordinate rice disease resistance and yield traits.
摘要：
活性氧（ROS）是水稻中的一组信号分子，在发育和胁迫反应的调节中起作用。呼吸爆发氧化酶同系物（Rbohs）是ROS产生的关键酶。然而,9个Rboh家族成员在水稻多病抗性和产量性状中的作用尚未完全了解。在这项研究中,我们构建了每个Rboh基因的突变体，并检测了它们在水稻多重抗病性和产量性状中的需求。我们的结果表明，五个Rboh基因(RbohA，RbohB,RbohE,RbohH,和RbohI）导致疾病苗圃和实验室条件下水稻稻瘟病抗性受损；五个Rbohs（RbohA，RbohB,RbohC,RbohE,和RbohH）导致在疾病苗圃和实验室条件下抑制水稻纹枯病抗性；六个Rbohs（RbohA，RbohB,RbohC,RbohE,RbohH和RbohI）导致稻田中水稻叶枯病抗性降低，以及PAMP和病原体诱导的ROS产生。此外，所有Rboh基因都参与水稻产量性状的调控,对于所有rboh突变体显示一个或多个受损的产量性状，例如圆锥花序数，每穗粒数，结实率，和谷物重量，导致单株产量降低，除了rbohb和rbohf。我们的结果确定了Rboh家族成员参与调节水稻对多种病原体的抗性，这些病原体引起了世界上最严重的疾病，并为这些Rboh在协调水稻抗病性和产量性状方面的育种应用提供了理论支持。
