Abstract：
Among the controversies in Lyme disease is the potential for Borrelia spirochetes to persist after guideline-directed antimicrobial therapy. Direct detection of the spirochetes has been essential to explore this phenomenon, given that the infection is often occult and infrequently observed in blood and other body fluids. In addition, the role of spirochetal infection has been examined in the etiology of neurodegenerative diseases through detection in affected tissues. In this chapter, we describe methodology to specifically identify Borrelia DNA, RNA, and intact organism (via protein) in tissue for studies of Lyme Borreliosis.
摘要：
莱姆病的争议之一是螺旋体螺旋体在指南指导的抗菌治疗后可能持续存在。螺旋体的直接检测对于探索这种现象至关重要，鉴于感染通常是隐匿性的，并且很少在血液和其他体液中观察到。此外,通过在受影响的组织中检测，已经检查了螺旋体感染在神经退行性疾病病因中的作用。在这一章中,我们描述了专门鉴定疏螺旋体DNA的方法，RNA,和组织中完整的生物体（通过蛋白质）用于莱姆病的研究。
