Abstract:
The cytopathic effect comprises the set of cellular alterations produced by a viral infection. It is of great relevance since it constitutes a direct marker of infection. Likewise, these alterations are often virus-specific which makes them a phenotypic marker for many viral species. All these characteristics have been used to complement the study of the dynamics of virus-cell interactions through the kinetic study of the progression of damage produced by the infection. Various approaches have been used to monitor the cytopathic effect, ranging from light microscopy, immunofluorescence assays, and direct labeling with fluorescent dyes, to plaque assay for the characterization of the infection over time. Here we address the relevance of the study of cytopathic effect and describe different experimental alternatives for its application.
摘要:
细胞病变效应包括由病毒感染产生的一组细胞改变。它具有很大的相关性,因为它构成了感染的直接标记。同样,这些改变通常是病毒特异性的,这使它们成为许多病毒物种的表型标记。通过对感染产生的损伤进展的动力学研究,所有这些特征已用于补充对病毒-细胞相互作用动力学的研究。各种方法已被用于监测细胞病变效应,从光学显微镜,免疫荧光测定,用荧光染料直接标记,随着时间的推移,用于表征感染的斑块测定。在这里,我们讨论了细胞病变效应研究的相关性,并描述了其应用的不同实验替代方案。