Abstract：
BACKGROUND: Rice is one of the most consumed cereals in the world. Productivity losses are caused by different biotic stresses. One of the most common is the phytophagous mite Schizotetranychus oryzae Rossi de Simons (Acari: Tetranychidae), which inhibits plant development and seed production. The identification of plant defense proteins is important for a better understanding of the mite-plant interaction. We previously detected a high expression of Osmotin1 protein in mite-resistant rice cultivars, under infested conditions, suggesting it could be involved in plant defense against mite attack. We therefore aimed to evaluate the responses of three rice lines overexpressing Osmotin1 (OSM1-OE) and three lines lacking the Osmotin1 gene (osm1-ko) to mite attack.
RESULTS: The numbers of individuals (adults, immature stages, and eggs) were significantly lower in OSM1-OE lines than those in wild-type (WT) plants. On the other hand, the osm1-ko lines showed larger numbers of mites per leaf than WT plants. When plants reached the full maturity stage, two out of the three infested OSM1-OE lines presented lower plant height than WT, while the three osm1-ko lines (infested or not) presented higher plant height than WT. The reduction in seed number caused by mite infestation was lower in OSM1-OE lines (12-19%) than in WT plants (34%), while osm1-ko lines presented higher reduction (24-54%) in seed number than WT plants (13%).
CONCLUSIONS: These data suggest that Osmotin1 is involved in rice resistance to S. oryzae infestation. This is the first work showing increased plant resistance to herbivory overexpressing an Osmotin gene. © 2023 Society of Chemical Industry.
RESULTS: The numbers of individuals (adults, immature stages, and eggs) were significantly lower in OSM1-OE lines than those in wild-type (WT) plants. On the other hand, the osm1-ko lines showed larger numbers of mites per leaf than WT plants. When plants reached the full maturity stage, two out of the three infested OSM1-OE lines presented lower plant height than WT, while the three osm1-ko lines (infested or not) presented higher plant height than WT. The reduction in seed number caused by mite infestation was lower in OSM1-OE lines (12-19%) than in WT plants (34%), while osm1-ko lines presented higher reduction (24-54%) in seed number than WT plants (13%).
CONCLUSIONS: These data suggest that Osmotin1 is involved in rice resistance to S. oryzae infestation. This is the first work showing increased plant resistance to herbivory overexpressing an Osmotin gene. © 2023 Society of Chemical Industry.
摘要：
背景：大米是世界上消费最多的谷物之一。生产力损失是由不同的生物胁迫引起的。最常见的一种是植食性螨米裂殖虫RossideSimons（Acari：Tetranychidae），抑制植物发育和种子生产。植物防御蛋白的鉴定对于更好地理解螨-植物相互作用是重要的。我们先前在抗螨水稻品种中检测到Osmotin1蛋白的高表达，在受感染的条件下，表明它可能参与植物防御螨攻击。因此,我们旨在评估过表达Osmotin1（OSM1-OE）的三个水稻品系和缺乏Osmotin1基因（osm1-ko）的三个品系对螨攻击的反应。
结果：个体数量(成人，不成熟的阶段，和卵）在OSM1-OE品系中显着低于野生型（WT）植物。另一方面,osm1-ko系的每叶螨虫数量比WT植物多。当植物达到完全成熟阶段时，三个受侵染的OSM1-OE系中有两个比WT高，而三个osm1-ko系（受感染或未感染）的株高高于WT。OSM1-OE系（12-19％）的螨侵染引起的种子数量减少低于WT植物（34％），而osM1-ko系的种子数量减少（24-54％）比WT植物（13％）更多。
结论：这些数据表明Osmotin1参与了水稻对米曲霉侵染的抗性。这是第一项工作，表明植物对草食性过表达Osmotin基因的抗性增加。本文受版权保护。保留所有权利。
结果：个体数量(成人，不成熟的阶段，和卵）在OSM1-OE品系中显着低于野生型（WT）植物。另一方面,osm1-ko系的每叶螨虫数量比WT植物多。当植物达到完全成熟阶段时，三个受侵染的OSM1-OE系中有两个比WT高，而三个osm1-ko系（受感染或未感染）的株高高于WT。OSM1-OE系（12-19％）的螨侵染引起的种子数量减少低于WT植物（34％），而osM1-ko系的种子数量减少（24-54％）比WT植物（13％）更多。
结论：这些数据表明Osmotin1参与了水稻对米曲霉侵染的抗性。这是第一项工作，表明植物对草食性过表达Osmotin基因的抗性增加。本文受版权保护。保留所有权利。
