Abstract：
Real-time vascular positioning, postoperative flap monitoring, and vascular reconstruction assessment are of great importance in flap transplantation. Cyanine dyes offer the advantage of high resolution in the Near-infrared-II (NIR-II) imaging window. However, the nonspecific binding of many cyanine dyes to endogenous albumin leads to high organ accumulation and skin absorption, resulting in low-quality imaging and poor reproducibility of contrast during long-term (e.g., 7 days) postoperative monitoring. Here, a novel strategy is proposed that can be widely applied to prevent protein binding for NIR-I/II Cl-containing cyanine dyes. This strategy produces protein-escaping dyes, ensuring high fluorescence enhancement in the blood with rapid clearance and no residual fluorescence, allowing for short-term repeatable injections for vascular imaging. This strategy in the perioperative monitoring of pedicle perforator flap models in mice and rats is successfully applied. Furthermore, leveraging the universality of this strategy, multiple nonoverlapping protein-escaping probes that achieve dual-excitation (808 and 1064 nm) interference-free imaging of nerve-vessel and tumor-vessel simultaneously are designed and synthesized. These protein-escaping dyes enable long-term repeatable dual-color imaging of tumor localization, resection, and tumor-vessel reconstruction at the wound site.
摘要：
实时血管定位,术后皮瓣监测,血管重建评估在皮瓣移植中具有重要意义。花青染料在NIR-II成像窗口中提供高分辨率的优点。然而,许多花青染料与内源性白蛋白的非特异性结合导致高器官积累和皮肤吸收，导致长期成像质量低且对比度再现性差(例如，7天)术后监测。这里,我们提出了一种新策略，可广泛应用于防止NIR-I/II含Cl的花青染料的蛋白质结合。这种策略产生蛋白质逃逸染料，确保血液中的高荧光增强，快速清除，没有残留荧光，允许短期可重复注射血管成像。我们已成功地将该策略应用于小鼠和大鼠带蒂穿支皮瓣模型的围手术期监测。此外，利用这一战略的普遍性，我们设计并合成了多个非重叠的蛋白质逃逸探针，可同时实现神经血管和肿瘤血管的双激发（808nm和1064nm）无干扰成像。这些蛋白质逃逸染料使肿瘤定位的长期可重复双色成像，切除,和伤口部位的肿瘤血管重建。本文受版权保护。保留所有权利。
