Abstract：
OBJECTIVE: The aim of the current study was to prepare a natural oral wound dressing from alginate modified with garden cress (GC), a rich source of antibacterial phytochemical compounds essential for wound healing.
METHODS: Sodium alginate (SA) dressing (negative control group), was prepared and modified with GC seeds extracts (25 µg/mL and 50 µg/mL) as the intervention groups, and COE-PAK was the positive control group. Cytotoxicity was measured using WST-1 assay (n = 15) after 24 and 48 hours. The in vitro wound healing assay (n = 15) was assessed in terms of wound width, and cell migration rate (0, 24, 48, and 72 hours). Agar diffusion test was performed to investigate the antibacterial action (n = 15) of the groups against Streptococcus mutans and Lactobacillus casei strains. Results were significant at p ≤ 0.05.
RESULTS: There was no statistically significant difference in cytotoxicity in all groups (p = 0.24 at 24 hours and 0.1 at 48 hours). Garden cress-containing groups revealed the lowest mean value of wound width (0.27 mm ± 0.01 and 0.23 mm ± 0.01 for 25 µg/mL and 50 µg/mL, respectively at 48 hours) and the highest mean value of cell migration rate (0.013 mm/hour ± 0.004 and 0.014 mm/hour ± 0.004 for 25 µg/mL and 50 µg/mL, respectively at 48 hours), in addition to the highest antibacterial action (1.49 mm ± 0.05 and 2.14 mm ± 0.09 for 25 µg/mL and 50 µg/mL, respectively against S. mutans, 1.43 mm ± 0.07 and 2.55 mm ± 0.09 for 25 µg/mL and 50 µg/mL, respectively against L. casei).
CONCLUSIONS: Alginate wound dressing modified with GC extract could be considered a promising wound dressing material in terms of wound healing and antibacterial action.
CONCLUSIONS: Ready-to-use alginate-based wound dressing modified with GC extract may represent a promising natural alternative to the most commonly used oral wound dressing (COE-PAK).
METHODS: Sodium alginate (SA) dressing (negative control group), was prepared and modified with GC seeds extracts (25 µg/mL and 50 µg/mL) as the intervention groups, and COE-PAK was the positive control group. Cytotoxicity was measured using WST-1 assay (n = 15) after 24 and 48 hours. The in vitro wound healing assay (n = 15) was assessed in terms of wound width, and cell migration rate (0, 24, 48, and 72 hours). Agar diffusion test was performed to investigate the antibacterial action (n = 15) of the groups against Streptococcus mutans and Lactobacillus casei strains. Results were significant at p ≤ 0.05.
RESULTS: There was no statistically significant difference in cytotoxicity in all groups (p = 0.24 at 24 hours and 0.1 at 48 hours). Garden cress-containing groups revealed the lowest mean value of wound width (0.27 mm ± 0.01 and 0.23 mm ± 0.01 for 25 µg/mL and 50 µg/mL, respectively at 48 hours) and the highest mean value of cell migration rate (0.013 mm/hour ± 0.004 and 0.014 mm/hour ± 0.004 for 25 µg/mL and 50 µg/mL, respectively at 48 hours), in addition to the highest antibacterial action (1.49 mm ± 0.05 and 2.14 mm ± 0.09 for 25 µg/mL and 50 µg/mL, respectively against S. mutans, 1.43 mm ± 0.07 and 2.55 mm ± 0.09 for 25 µg/mL and 50 µg/mL, respectively against L. casei).
CONCLUSIONS: Alginate wound dressing modified with GC extract could be considered a promising wound dressing material in terms of wound healing and antibacterial action.
CONCLUSIONS: Ready-to-use alginate-based wound dressing modified with GC extract may represent a promising natural alternative to the most commonly used oral wound dressing (COE-PAK).
摘要：
目的：本研究的目的是制备一种天然的口腔伤口敷料，该敷料是由海藻酸盐修饰的花园豆瓣菜（GC），对伤口愈合至关重要的抗菌植物化学物质的丰富来源。
方法：海藻酸钠（SA）敷料（阴性对照组），用GC种子提取物（25µg/mL和50µg/mL）作为干预组进行制备和修饰，阳性对照组为COE-PAK。在24和48小时后使用WST-1测定(n=15)测量细胞毒性。根据伤口宽度评估体外伤口愈合测定（n=15），和细胞迁徙率（0、24、48和72小时）。进行琼脂扩散测试以研究该组对变形链球菌和干酪乳杆菌菌株的抗菌作用（n=15）。结果在p≤0.05时显著。
结果：所有组的细胞毒性没有统计学上的显着差异（24小时时p=0.24，48小时时p=0.1）。含有花园水芹的组显示伤口宽度的平均值最低（对于25µg/mL和50µg/mL，为0.27mm±0.01和0.23mm±0.01，分别在48小时）和细胞迁移速率的最高平均值（对于25µg/mL和50µg/mL，0.013mm/小时±0.004和0.014mm/小时±0.004，分别在48小时)，除了最高的抗菌作用（对于25µg/mL和50µg/mL，1.49mm±0.05和2.14mm±0.09，分别针对S.mutans，对于25µg/mL和50µg/mL，1.43mm±0.07和2.55mm±0.09，分别针对L.Casei）。
结论：用GC提取物修饰的海藻酸盐伤口敷料在伤口愈合和抗菌作用方面被认为是一种有前途的伤口敷料材料。
结论：用GC提取物修饰的即用藻酸盐基伤口敷料可能是最常用的口腔伤口敷料（COE-PAK）的有希望的天然替代品。
方法：海藻酸钠（SA）敷料（阴性对照组），用GC种子提取物（25µg/mL和50µg/mL）作为干预组进行制备和修饰，阳性对照组为COE-PAK。在24和48小时后使用WST-1测定(n=15)测量细胞毒性。根据伤口宽度评估体外伤口愈合测定（n=15），和细胞迁徙率（0、24、48和72小时）。进行琼脂扩散测试以研究该组对变形链球菌和干酪乳杆菌菌株的抗菌作用（n=15）。结果在p≤0.05时显著。
结果：所有组的细胞毒性没有统计学上的显着差异（24小时时p=0.24，48小时时p=0.1）。含有花园水芹的组显示伤口宽度的平均值最低（对于25µg/mL和50µg/mL，为0.27mm±0.01和0.23mm±0.01，分别在48小时）和细胞迁移速率的最高平均值（对于25µg/mL和50µg/mL，0.013mm/小时±0.004和0.014mm/小时±0.004，分别在48小时)，除了最高的抗菌作用（对于25µg/mL和50µg/mL，1.49mm±0.05和2.14mm±0.09，分别针对S.mutans，对于25µg/mL和50µg/mL，1.43mm±0.07和2.55mm±0.09，分别针对L.Casei）。
结论：用GC提取物修饰的海藻酸盐伤口敷料在伤口愈合和抗菌作用方面被认为是一种有前途的伤口敷料材料。
结论：用GC提取物修饰的即用藻酸盐基伤口敷料可能是最常用的口腔伤口敷料（COE-PAK）的有希望的天然替代品。
